RA10109-50 P2X3 antibody
see related secondary antibodiessee all 13 P2X3 products
50 µl / no delivery possible
Quick Overview
Rabbit anti P2X3
Synonyms
P2X purinoceptor 3, Purinergic receptor, P2RX3,
Product Description
Rabbit anti P2X3 , Presentation: Serum. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ), Western blot / Immunoblot ( WB ) |
| Reactivity | Rat ( Rt ), Human ( Hu ), Monkey ( Mky ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | RA10109-50 |
| Quantity | 50 µl |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Neuromics Antibodies |
| Datasheet | view  RA10109.pdf |
Datasheet Extract
| Immunogen | Corresponding to residues 383-397 of the carboxy-terminus of rat P2X3. AA Sequence: VEKQSTDSGAYSIGH |
| Format | State: Liquid serum containing 0.05% sodium azide |
| Applications | Immunohistochemistry: 1:1000.
Immunocytochemistry: 1:1000. Western Blot: 1:1000. |
| Specificity | This antibody reacts to P2X3. Species: Rat, Primate, Human. |
| Add. Information | Sodium azide (NaN3) interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide-free buffer before performing the peroxidase reaction. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: 6 month from despatch. |
| References | 1. Chen, C. C., Akopian, A. N., Sivilotti, L., Colquhoun, D., Burnstock, G., and Wood, J. N. (1995). A P2X purinoceptor expressed by a sugset of sensory neurons [see comments]. Nature 377, 428-31.
2. Lewis, C., Neidhart, S., Holy, C., North, R. A., Buell, G., and Surprenant, A. (1995). Coexpression of P2X2 and P2X3 receptor subunits can account for ATP-gated currents in sensory neurons [see comments]. Nature 377, 432-5. 3. Vulchanova, L., Riedl, M. S., Shuster, S. J., Buell, G., Surprenant, A., North, R. A., and Elde, R. (1997). Immunohistochemical study of the P2X2 and P2X3 receptor subunits in rat and monkey sensory neurons and their central terminals. Neuropharmacology 36, 1229-42. 4. Vulchanova, L., Riedl, M. S., Shuster, S. J., Stone, L. S., Hargreaves, K. M., Buell, G., Surprenant, A., North, R. A., and Elde, R. (1998). P2X3 is expressed by DRG neurons that terminate in inner lamina II. Eur J Neurosci 10, 3470-8. |
| Protocols | Immunohistochemistry:
Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrodes solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Immunocytochemistry: P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above. Western Blotting: Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 4°C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated with primary antiserum (1:500 in the same buffer overnight at 4°C. Membranes were rinsed and incubated with horseradish peroxidase conjugated secondary antibody for 1 hour at room temperature. Following rinsing, the membranes were processed using enhanced chemiluminescence. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'RA10109-50 P2X3'.
