RA10107-50 P2X1 antibody
see related secondary antibodiessee all 5 P2X1 products
50 µl / no delivery possible
Quick Overview
Rabbit anti P2X1
Synonyms
P2X purinoceptor 1, Purinergic receptor, P2RX1,
Product Description
Rabbit anti P2X1 , Presentation: Serum. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ) |
| Reactivity | Rat ( Rt ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | RA10107-50 |
| Quantity | 50 µl |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Neuromics Antibodies |
| Datasheet | view  RA10107.pdf |
Datasheet Extract
| Immunogen | Corresponding to residues 364-372 of the rat P2X1. AA Sequence: GLQENMRTS |
| Format | State: Liquid serum containing 0.05% sodium azide |
| Applications | Immunohistochemistry: 1:1000.
Immunocytochemistry: 1:1000. |
| Specificity | This antibody reacts to P2X1. Species: Rat. |
| Add. Information | Sodium azide (NaN3) interferes with peroxidase reactions and should not be used with peroxidase methodologies. If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide-free buffer before performing the peroxidase reaction. |
| Storage | Store the antibody at -20°C.
Avoid repeated freezing and thawing. Shelf life: 6 month from despatch. |
| References | 1. Valera, S., Hussy, N., Evans, R. J., Adami, N., North, R.. A., Surprenant, A., and Buell, G. (1994). A new class o ligand-gaed ion channel defined by P2X receptor for extracellula ATP Nature 371, 516 - 9.
2. Vulchanova, L., Arvidsson, U., Riedl, M., Wang, J., Buell, G., Surprenant, A., North, R. A., and Elde, R. (1996). Differential distribution of two ATP-gated channels (P2X receptors) determined by immunocytochemistry. Proc Natl Acad Sci U S A 93, 8063 - 7. |
| Protocols | Immunohistochemistry:
Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrodes solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. Immunocytochemistry: P2X1 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'RA10107-50 P2X1'.
