R1572P DNA Methyltransferase 3-like (DNMT3L) antibody
see related secondary antibodiessee all 4 DNA Methyltransferase 3-like products
0.1 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti DNA Methyltransferase 3-like (DNMT3L) -
Synonyms
Dnmt-like DNA -methyltransferase 3-like MGC1090 cytosine-5-
Product review
Please read our product review about DNA Methyltransferase 3-like (DNMT3L): Antibodies to DNA methyltransferases (Dnmt Family).
Product Description
Rabbit anti DNA Methyltransferase 3-like (DNMT3L) -, Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | R1572P |
| Quantity | 0.1 mg |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1572P.pdf |
Datasheet Extract
| Polyclonal antibody to DNMT3L //(DNA (cytosine-5-)-methyltransferase 3-like | |
| Concentration | 1.13 mg/ml (by UV absorbance at 280 nm) Format: This antibody is supplied as sterile filtered, imunoaffinity purified liquid Ig fraction in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as |
| Background | DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. |
| Immunogen | A synthetic peptide corresponding to aa 152-164 of human DNMT3L. AA Sequence: Peptide: G-L-L-Q-R-R-R-K-W-R-S-Q-L Protein: 1 maaipaldpe aepsmdvilv gsselsssvs pgtgrdliay evkanqrnie diciccgslq 61 vhtqhplfeg gicapckdkf ldalflyddd gyqsycsicc sgetllicgn pdctrcycfe 121 cvdslvgpgt sgkvhamsnw vcylclpssr sgllqrrrkw rsqlkafydr esenplemfe 181 tvpvwrrqpv rvlslfedik keltslgfle sgsdpgqlkh vvdvtdtvrk dveewgpfdl 241 vygatpplgh tcdrppswyl fqfhrllqya rpkpgspgpf fwmfvdnlvl nkedldvasr 301 flemepvtip dvhggslqna vrvwsnipai rsrhwalvse eelsllaqnk qssklaakwp 361 tklvkncflp lreyfkyfst eltssl |
| Format | State: Liquid (sterile filtered) Ig fraction Purification: Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide |
| Applications | This product has been assayed by ELISA, western blotting and immunoprecipitation.
The antibody was tested by ELISA against 0.1 ug of the immunizing peptide. A 1:1,000 to 1:3,000 dilution of the antibody is recommended for this assay. For western blotting, first immunoprecipitate the antigen using this antibody and then detect using by western blotting using a 1:1,500 dilution of the antibody. See Fuks et al. (2000)for additional details. The antibody does not perform well when applied directly for western blotting. The predicted molecular weight of DNMT3L is 43.6 kDa. |
| Specificity | This antibody reacts with DNMT3L. Species: Human. This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. |
| Storage | Store vial at -20° C prior to opening. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | Suetake I, Shinozaki F, Miyagawa J, Takeshima H, Tajima S. (2004) DNMT3L stimulates the DNA methylation activity of Dnmt3a and Dnmt3b through a direct interaction. J Biol Chem. Apr 21 [Epub ahead of print]
Lucifero D. et al. (2004) Gene-specific timing and epigenetic memory in oocyte imprinting. Hum Mol Genet. 13(8):839-49. Gribnau J, Hochedlinger K, Hata K, Li E, Jaenisch R. (2003) Asynchronous replication timing of imprinted loci is independent of DNA methylation, but consistent with differential subnuclear localization. Genes Dev. 17(6):759-73. Chedin F, Lieber MR, Hsieh CL. (2002) The DNA methyltransferase-like protein DNMT3L stimulates de novo methylation by Dnmt3a. Proc Natl Acad Sci U S A. 99(26):16916-21. Deplus R. et al. (2002) Dnmt3L is a transcriptional repressor that recruits histone deacetylase. Nucleic Acids Res. 30(17):3831-8. Aapola U, Liiv I, Peterson P. (2002) Imprinting regulator DNMT3L is a transcriptional repressor associated with histone deacetylase activity. Nucleic Acids Res. 30(16):3602-8. Bourc'his D, Xu GL, Lin CS, Bollman B, Bestor TH. (2001) Dnmt3L and the establishment of maternal genomic imprints. Science. 294(5551):2536-9. Aapola U, Lyle R, Krohn K, Antonarakis SE, Peterson P. (2001) Isolation and initial characterization of the mouse Dnmt3l gene. Cytogenet Cell Genet. 92(1-2):122-6. Aapola U. et al. (2000) Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family. Genomics. 65(3):293-8. Fuks F, Burgers WA, Brehm A, Hughes-Davies L, Kouzarides T.(2000) DNA methyltransferase Dnmt1 associates with histone deacetylase activity. Nat Genet. 24(1):88-91. |
| Pictures | Immunoblotting: Affinity Purified Rabbit anti-DNMT3L was used at a 1:1,500 dilution to detect human DNMT3L by western blot after
immunoprecipitation using the same antibody. Transfection of U2OS cells (10cm dish) was accomplished using 3 μg of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For IP and WB conditions see Fuks et al. (2000). For western blotting the antibody was used at 0.4 μg/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL. Note: this antibody works for western blotting only following immunoprecipitation. We have not had good success using the antibody directly for western blotting. The expected molecular weight of human DNMT3L is 43.6 kDa. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1572P DNA Methyltransferase 3-like (DNMT3L)'.
