R1521 p19/Skp1 (C-term.) antibody
see related secondary antibodiessee all 2 p19/Skp1 products
0.1 ml / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti p19/Skp1 (C-term.)
Synonyms
p19Skp1,
Product Description
Rabbit anti p19/Skp1 (C-term.) , Presentation: Serum. Product is tested for Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | R1521 |
| Quantity | 0.1 ml |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1521.pdf |
Datasheet Extract
| Alternate names | S-phase kinase-associated protein 1A, Cyclin A/CDK2-associated protein p19, RNA polymerase II elongation factor-like protein, OCP-II protein |
| Concentration | 85.0 mg/ml (by Refractometry) |
| Background | SKP1 is also known as S-phase kinase-associated protein 1A, Cyclin A/CDK2-associated protein p19, p19A, p19skp1, RNA polymerase II elongation factor-like protein, Organ of Corti protein 2, OCP-II protein, OCP-2, Transcription elongation factor B, and SIII. SKP1 is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, SKP1 serves as an adapter that links the F-box protein to CUL1. SKP1 interacts directly with CUL1 and F-box proteins, such as BTRC and SKP2, in the SCF complex. SKP1 also interacts with FBX29 and the cyclin A/CDK2 complex. SKP1 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8 and is also a component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X. |
| Immunogen | Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 152-163 of Human SKP1 (C-terminus) coupled to KLH. AA Sequence: Human SKP1 (163 aa 18.5 kDa) 1 mpsiklqssd geifevdvei akqsvtiktm ledlgmddeg dddpvplpnv naailkkviq 61 wcthhkddpp ppeddenkek rtddipvwdq eflkvdqgtl felilaanyl dikglldvtc 121 ktvanmikgk tpeeirktfn ikndfteeee aqvrkenqwc eek |
| Format | State: Liquid (sterile filtered) with 0.01% (w/v) Sodium Azide as preservative. Purification: Delipidation and defibrination. |
| Applications | ELISA (1/2,000-1/10,000). This antibody reacts with human SKP1 by Western blot (1/500-1/1,000) and Immunoprecipitation. The antibody Immunoprecipitates in vitro translated protein and protein from cell lysates (using HeLa, NIH-3T3, and others). Coimmunoprecipitation of cyclin A and Cuol1 may occur. An 18.5 kDa band corresponding to human SKP1 is detected. Most cell lines expressing SKP1 can be used as a positive control. |
| Description | AASequence: Human SKP1 (163 aa 18.5 kDa) 1 mpsiklqssd geifevdvei akqsvtiktm ledlgmddeg dddpvplpnv naailkkviq 61 wcthhkddpp ppeddenkek rtddipvwdq eflkvdqgtl felilaanyl dikglldvtc 121 ktvanmikgk tpeeirktfn ikndfteeee aqvrkenqwc eek Related Link(s): Accession # P63208 or P63208. Specific Reference(s): Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449. Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541. Zhang,H., Kobayashi,R., Galaktionov,K. and Beach,D. (1995) p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase. Cell 82 (6), 915-925. Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ. (1996) SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell. 26;86(2):263-74. Connelly C, Hieter P. (1996) Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression. Cell. 86(2):275-85. Feldman RM, Correll CC, Kaplan KB, Deshaies RJ. (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell. 91(2):221-30. Liu J, Furukawa M, Matsumoto T, Xiong Y. (2002) NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell. (6):1511-8. General Reference(s): Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42. Sowden,J., Morrison,K., Schofield,J., Putt,W. and Edwards,Y. (1995) A novel cDNA with homology to an RNA polymerase II elongation factor maps to human chromosome 5q31 (TCEB1L) and to mouse chromosome 11 (Tceb1l). Genomics 29 (1), 145-151. Liang,Y., Chen,H., Asher,J.H. Jr., Chang,C.C. and Friedman,T.B. (1997) Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qte. Gene 184 (2), 163-167. Dias,D.C., Dolios,G., Wang,R. and Pan,Z.Q. (2002) CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex. Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16601-16606. Matsuzawa,S.I. and Reed,J.C. (2001) Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses. Mol. Cell 7 (5), 915-926. Schulman,B.A., Carrano,A.C., Jeffrey,P.D., Bowen,Z., Kinnucan,E.R., Finnin,M.S., Elledge,S.J., Harper,J.W., Pagano,M. and Pavletich,N.P. (2000) Insights into SCF ubiquitin |
| Specificity | This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
This product reacts with human SKP1. Cross reactivity is expected against mouse SKP1 based on a high degree of sequence homology. Cross reactivity with other SKP1 proteins or SKP1 from other sources is not known. |
| Storage | Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
| References | Specific Reference(s):
1. Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449. 2. Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541. 3. Zhang,H., Kobayashi,R., Galaktionov,K. and Beach,D. (1995) p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase. Cell 82 (6), 915-925. 4. Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ. (1996) SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell. 26;86(2):263-74. 5. Connelly C, Hieter P. (1996) Budding yeast SKP1 encodes an evolutionarily conserved kinetochore protein required for cell cycle progression. Cell. 86(2):275-85. 6. Feldman RM, Correll CC, Kaplan KB, Deshaies RJ. (1997) A complex of Cdc4p, Skp1p, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Sic1p. Cell. 91(2):221-30. 7. Liu J, Furukawa M, Matsumoto T, Xiong Y. (2002) NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases. Mol Cell. (6):1511-8. General Reference(s): 1. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42. 2. Sowden,J., Morrison,K., Schofield,J., Putt,W. and Edwards,Y. (1995) A novel cDNA with homology to an RNA polymerase II elongation factor maps to human chromosome 5q31 (TCEB1L) and to mouse chromosome 11 (Tceb1l). Genomics 29 (1), 145-151. 3. Liang,Y., Chen,H., Asher,J.H. Jr., Chang,C.C. and Friedman,T.B. (1997) Human inner ear OCP2 cDNA maps to 5q22-5q35.2 with related sequences on chromosomes 4p16.2-4p14, 5p13-5q22, 7pter-q22, 10 and 12p13-12qte. Gene 184 (2), 163-167. 4. Dias,D.C., Dolios,G., Wang,R. and Pan,Z.Q. (2002) CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex. Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16601-16606. 5. Matsuzawa,S.I. and Reed,J.C. (2001) Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses. Mol. Cell 7 (5), 915-926. 6. Schulman,B.A., Carrano,A.C., Jeffrey,P.D., Bowen,Z., Kinnucan,E.R., Finnin,M.S., Elledge,S.J., Harper,J.W., Pagano,M. and Pavletich,N.P. (2000) Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex. Nature 408 (6810), 381-386. |
| Pictures | Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references above. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1521 p19/Skp1 (C-term.)'.
