R1514 Cullin-7 / CUL-7 (C-term) antibody
see related secondary antibodiessee all 8 Cullin-7 / CUL-7 products
0.1 ml / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Cullin-7 / CUL-7 -
Synonyms
Cullin 7, Cullin7, CUL7, KIAA0076, , Cullin homolog 7, CUL 7, p185, p180, dJ20C7.5
Product review
Please read our product review about Cullin-7 / CUL-7 : Antibodies to Cullin Protein Family.
Product Description
Rabbit anti Cullin-7 / CUL-7 -, Presentation: Serum. Product is tested for Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | R1514 |
| Swiss Prot. No. | Q14999 |
| Quantity | 0.1 ml |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1514.pdf |
Datasheet Extract
| Polyclonal Antibody to Human Cullin 7// (C-terminal specific) | |
| Concentration | 85.0 mg/ml (by Refractometry) |
| Background | Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone. |
| Immunogen | Synthetic peptide corresponding to amino acids 1679-1698 of Human Cul7 (C-terminus) coupled coupled to KLH. Swiss Num.: Q14999 |
| Format | State: Liquid (sterile filtered) containing 0.01% (w/v) Sodium Azide as preservative. Purification: Delipidation and defibrination. Purity: and |
| Applications | Western blot (1/500-1/1,000): This antibody reacts with human Cul7, An 191.2 kDa band corresponding to human Cul7 is detected; Most cell lines expressing Cul7 can be used as a positive control.
Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein and also protein from cell lysates (using HeLa or NIH-3T3 and others. This antibody also co-immunoprecipitates associated proteins in overexpressed CUL7 systems). ELISA: 1/2,000-1/10,000 Dilution. |
| Description | AASequence: Human Cullin 7 (1698 aa 191.2kDa) 1 mvgelryref rvplgpglha ypdelirqrv ghdghpeyqi rwlilrrgde gdggsgqvdc 61 kaehillwms kdeiyanchk mlgedgqvig psqesagevg aldksvleem etdvksliqr 121 alrqleecvg tippapllht vhvlsayasi epltgvfkdp rvldllmhml sspdyqirws 181 agrmiqalss hdagtrtqil lslsqqeaie khldfdsrca llalfaqatl sehpmsfegi 241 qlpqvpgrvl fslvkrylhv tslldqlnds aaepgaqnts apeelsgerg qlelefsmam 301 gtliselvqa mrwdqasdrp rssarspgsi fqpqladvsp glpaaqaqps frrsrrfrpr 361 sefasgntya lyvrdtlqpg mrvrmlddye eisagdegef rqsnngvppv qvfwestgrt 421 ywvhwhmlei lgfeediedm veadeyqgav asrvlgralp awrwrpmtel yavpyvlped 481 edteecehlt laewwellff ikkldgpdhq evlqilqenl dgeilddeil aelavpiela 541 qdllltlpqr lndsalrdli nchvykkygp ealagnqayp slleaqedvl lldaqaqakd 601 sedaakveak eppsqspntp lqrlvegygp agkilldleq alssegtqen kvkplllqlq 661 rqpqpflalm qsldtpetnr tlhltvlril kqlvdfpeal llpwheavda cmaclrspnt 721 drevlqelif flhrltsvsr dyavvlnqlg ardaiskale khlgklelaq elrdmvfkce 781 khahlyrkli tnilggciqm vlgqiedhrr thqpinipff dvflrylcqg ssvevkedkc 841 wekvevssnp hraskltdhn pktywesngs agshyitlhm rrgilirqlt llvasedssy 901 mparvvvcgg dstsslhtel nsvnvmpsas rvillenltr fwpiiqirik rcqqggidtr 961 irgleilgpk ptfwpvfreq lcrhtrlfym vraqawsqdm aedrrsllhl ssrlngalrq 1021 eqnfadrflp ddeaaqalgk tcwealvspv vqnitspded gisplgwlld qylecqeavf 1081 npqsrgpaff srvrrlthll vhvepceapp pvvatprpkg rnrshdwssl atrglpssim 1141 rnltrcwrav vekqvnnflt sswrdddfvp rycehfnilq nssselfgpr aafllalqng 1201 cagallklpf lkaahvseqf arhidqqiqg sriggaqeme rlaqlqqclq avlifsglei 1261 attfehyyqh ymadrllgvv sswlegavle qigpcfpnrl pqqmlqslst skelqrqfhv 1321 yqlqqldqel lkledtekki qvglgasgke hksekeeeag aaavvdvaeg eeeeeenedl 1381 yyegampevs vlvlsrhswp vasichtlnp rtclpsylrg tlnrysnfyn ksqshpaler 1441 gsqrrlqwtw lgwaelqfgn qtlhvstvqm wlllylndlk avsvesllaf sglsadmlnq 1501 aigpltssrg pldlheqkdi pggvlkirdg skeprsrwdi vrlippqtyl qaegedgqnl 1561 ekrrnllncl ivrilkahgd eglhidqlvc lvleawqkgp cpprglvssl gkgsacsstd 1621 vlscilhllg kgtlrrhddr pqvlsyavpv tvmephtesl npgssgpnpp ltfhtlqirs 1681 rgvpyascta tqsfstfr Specific |
| Specificity | This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
This product reacts with human and mouse Cullin 7. Cross reactivity with other human cullins may occur. |
| Add. Information | Protein Sequence: Human Cullin 7 (1698 aa 191.2kDa):
1 mvgelryref rvplgpglha ypdelirqrv ghdghpeyqi rwlilrrgde gdggsgqvdc 61 kaehillwms kdeiyanchk mlgedgqvig psqesagevg aldksvleem etdvksliqr 121 alrqleecvg tippapllht vhvlsayasi epltgvfkdp rvldllmhml sspdyqirws 181 agrmiqalss hdagtrtqil lslsqqeaie khldfdsrca llalfaqatl sehpmsfegi 241 qlpqvpgrvl fslvkrylhv tslldqlnds aaepgaqnts apeelsgerg qlelefsmam 301 gtliselvqa mrwdqasdrp rssarspgsi fqpqladvsp glpaaqaqps frrsrrfrpr 361 sefasgntya lyvrdtlqpg mrvrmlddye eisagdegef rqsnngvppv qvfwestgrt 421 ywvhwhmlei lgfeediedm veadeyqgav asrvlgralp awrwrpmtel yavpyvlped 481 edteecehlt laewwellff ikkldgpdhq evlqilqenl dgeilddeil aelavpiela 541 qdllltlpqr lndsalrdli nchvykkygp ealagnqayp slleaqedvl lldaqaqakd 601 sedaakveak eppsqspntp lqrlvegygp agkilldleq alssegtqen kvkplllqlq 661 rqpqpflalm qsldtpetnr tlhltvlril kqlvdfpeal llpwheavda cmaclrspnt 721 drevlqelif flhrltsvsr dyavvlnqlg ardaiskale khlgklelaq elrdmvfkce 781 khahlyrkli tnilggciqm vlgqiedhrr thqpinipff dvflrylcqg ssvevkedkc 841 wekvevssnp hraskltdhn pktywesngs agshyitlhm rrgilirqlt llvasedssy 901 mparvvvcgg dstsslhtel nsvnvmpsas rvillenltr fwpiiqirik rcqqggidtr 961 irgleilgpk ptfwpvfreq lcrhtrlfym vraqawsqdm aedrrsllhl ssrlngalrq 1021 eqnfadrflp ddeaaqalgk tcwealvspv vqnitspded gisplgwlld qylecqeavf 1081 npqsrgpaff srvrrlthll vhvepceapp pvvatprpkg rnrshdwssl atrglpssim 1141 rnltrcwrav vekqvnnflt sswrdddfvp rycehfnilq nssselfgpr aafllalqng 1201 cagallklpf lkaahvseqf arhidqqiqg sriggaqeme rlaqlqqclq avlifsglei 1261 attfehyyqh ymadrllgvv sswlegavle qigpcfpnrl pqqmlqslst skelqrqfhv 1321 yqlqqldqel lkledtekki qvglgasgke hksekeeeag aaavvdvaeg eeeeeenedl 1381 yyegampevs vlvlsrhswp vasichtlnp rtclpsylrg tlnrysnfyn ksqshpaler 1441 gsqrrlqwtw lgwaelqfgn qtlhvstvqm wlllylndlk avsvesllaf sglsadmlnq 1501 aigpltssrg pldlheqkdi pggvlkirdg skeprsrwdi vrlippqtyl qaegedgqnl 1561 ekrrnllncl ivrilkahgd eglhidqlvc lvleawqkgp cpprglvssl gkgsacsstd 1621 vlscilhllg kgtlrrhddr pqvlsyavpv tvmephtesl npgssgpnpp ltfhtlqirs 1681 rgvpyascta tqsfstfr |
| Storage | Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Arai,T., Kasper,J.S., Skaar,J.R., Ali,S.H., Takahashi,C. and DeCaprio,J.A. (2003) Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis. Proc. Natl. Acad. Sci. U.S.A. 100 (17), 9855-9860.
2. Dias,D.C., Dolios,G., Wang,R. and Pan,Z.Q. (2002) CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex. Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16601-16606. 3. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42. |
| Pictures | Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs).
Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references above. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1514 Cullin-7 / CUL-7 '.