R1512 Cullin-4A / CUL-4A (N-term) antibody
see related secondary antibodies
0.1 ml / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Cullin-4A / CUL-4A -
Synonyms
Cullin 4A, Cullin4A, CUL4A, CUL 4A, ,
Product review
Please read our product review about Cullin-4A / CUL-4A: Antibodies to Cullin Protein Family.
Product Description
Rabbit anti Cullin-4A / CUL-4A -, Presentation: Serum. Product is tested for Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | R1512 |
| Swiss Prot. No. | Q13619 |
| Quantity | 0.1 ml |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1512.pdf |
Datasheet Extract
| Polyclonal Antibody to Human Cul4A //(N-terminal specific) | |
| Alternate names | Cullin 4A |
| Concentration | 85.0 mg/ml (by Refractometry) |
| Background | Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 4a is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul4A serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul4A may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul4A also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1. |
| Immunogen | Synthetic peptide corresponding to amino acids 1-20 of Human Cul4A (N-terminus) coupled coupled to KLH. Swiss Num.: Q13619 AA Sequence: Human Cullin 4A (759 aa 87.6 kDa): 1 madeaprkgs fsalvgrtng ltkpaalaaa pakpggaggs kklviknfrd rprlpdnytq 61 dtwrklheav ravqsstsir ynleelyqav enlcshkvsp mlykqlrqac edhvqaqilp 121 fredsldsvl flkkintcwq dhcrqmimir siflfldrty vlqnstlpsi wdmglelfrt 181 hiisdkmvqs ktidgillli erersgeavd rsllrsllgm lsdlqvykds felkfleetn 241 clyaaegqrl mqerevpeyl nhvskrleee gdrvityldh stqkpliacv ekqllgehlt 301 ailqkgldhl ldenrvpdla qmyqlfsrvr ggqqallqhw seyiktfgta ivinpekdkd 361 mvqdlldfkd kvdhvievcf qknerfvnlm kesfetfink rpnkpaelia khvdsklrag 421 nkeatdeele rtldkimilf rfihgkdvfe afykkdlakr llvgksasvd aeksmlsklk 481 hecgaaftsk legmfkdmel skdimvhfkq hmqnqsdsgp idltvniltm gywptytpme 541 vhltpemikl qevfkafylg khsgrklqwq ttlghavlka efkegkkefq vslfqtlvll 601 mfnegdgfsf eeikmatgie dselrrtlqs lacgkarvli kspkgkeved gdkfifngef 661 khklfrikin qiqmketvee qvsttervfq drqyqidaai vrimkmrktl ghnllvsely 721 nqlkfpvkpg dlkkriesli drdymerdkd npnqyhyva |
| Format | State: Liquid serum (sterile filtered) containing 0.01% (w/v) Sodium Azide as preservative. Purification: Delipidation and defibrination. Purity: and |
| Applications | Western blot: This antibody reacts with human Cul4A (1/500-1/1,000; An 87.6 kDa band corresponding to human Cul4A is detected; Most cell lines expressing Cul4A can be used as a positive control).
Immunoprecipitation: The antibody immunoprecipitates protein from cell lysates (using HeLa or U2OS and others). ELISA (1/2,000-1/10,000). |
| Description | AASequence: Human Cullin 4A (759 aa 87.6 kDa) 1 madeaprkgs fsalvgrtng ltkpaalaaa pakpggaggs kklviknfrd rprlpdnytq 61 dtwrklheav ravqsstsir ynleelyqav enlcshkvsp mlykqlrqac edhvqaqilp 121 fredsldsvl flkkintcwq dhcrqmimir siflfldrty vlqnstlpsi wdmglelfrt 181 hiisdkmvqs ktidgillli erersgeavd rsllrsllgm lsdlqvykds felkfleetn 241 clyaaegqrl mqerevpeyl nhvskrleee gdrvityldh stqkpliacv ekqllgehlt 301 ailqkgldhl ldenrvpdla qmyqlfsrvr ggqqallqhw seyiktfgta ivinpekdkd 361 mvqdlldfkd kvdhvievcf qknerfvnlm kesfetfink rpnkpaelia khvdsklrag 421 nkeatdeele rtldkimilf rfihgkdvfe afykkdlakr llvgksasvd aeksmlsklk 481 hecgaaftsk legmfkdmel skdimvhfkq hmqnqsdsgp idltvniltm gywptytpme 541 vhltpemikl qevfkafylg khsgrklqwq ttlghavlka efkegkkefq vslfqtlvll 601 mfnegdgfsf eeikmatgie dselrrtlqs lacgkarvli kspkgkeved gdkfifngef 661 khklfrikin qiqmketvee qvsttervfq drqyqidaai vrimkmrktl ghnllvsely 721 nqlkfpvkpg dlkkriesli drdymerdkd npnqyhyva Specific |
| Specificity | This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
This product reacts with human Cullin 4A. Cross reactivity is expected against mouse Cul4A based on a high degree of sequence homology. Cross reactivity with other human cullins may occur. |
| Storage | Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Higa,L.A., Mihaylov,I.S., Banks,D.P., Zheng,J. and Zhang,H. (2003) Radiation-mediated proteolysis of CDT1 by CUL4-ROC1 and CSN complexes constitutes a new checkpoint. Nat. Cell Biol. 5 (11), 1008-1015.
2. Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839. 3. Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449. 4. Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541. 5. Mathias, N., Johnson, S.L., Winey, M., Adams, A.E., Goetsch, L., Pringle, J.R., Byers, B., Goebl, M.G. (1996) Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins. Mol Cell Biol 16:12, 6634-6643. 6. Groisman R, Polanowska J, Kuraoka I, Sawada J, Saijo M, Drapkin R, Kisselev AF, Tanaka K, Nakatani Y. (2003) The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. Cell. 113(3):357-67. 7. Li B, Ruiz JC, Chun KT. (2002) CUL-4A is critical for early embryonic development. Mol Cell Biol. 22(14):4997-5005. 8. Shiyanov P, Nag A, Raychaudhuri P. (1999) Cullin 4A associates with the UV-damaged DNA-binding protein DDB. J Biol Chem. 274(50):35309-12. 9. Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-42. 10. Chen L.-C., Manjeshwar S., Lu Y., Moore D., Ljung B.M., Kuo W.L., Dairkee S.H., Wernick M., Collins C., Smith H.S.(1998) The human homologue for the Caenorhabditis elegans cul-4 gene is amplified and overexpressed in primary breast cancers. Cancer Res. 58:3677-3683. |
| Pictures | Figure 1. Conjugation pathways for ubiquitin and
ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin- 2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references above. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1512 Cullin-4A / CUL-4A'.