R1511 Cullin-3 (CUL-3) (N-term.) antibody
see related secondary antibodiessee all 10 Cullin-3 products
0.1 ml / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Cullin-3 (CUL-3) (N-term.) -
Synonyms
Cullin 3 CUL3
Product review
Please read our product review about Cullin-3 (CUL-3) (N-term.): Antibodies to Cullin Protein Family.
Product Description
Rabbit anti Cullin-3 (CUL-3) (N-term.) -, Presentation: Serum. Product is tested for Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | R1511 |
| Quantity | 0.1 ml |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1511.pdf |
Datasheet Extract
| Polyclonal Antibody to Human Cul3// (N-terminal specific) | |
| Alternate names | Cullin 3 |
| Concentration | 85.0 mg/ml (by Refractometry) |
| Background | Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1. |
| Immunogen | Synthetic peptide corresponding to amino acids 1-12 of Human Cul3 (N-terminus) coupled to KLH. AA Sequence: Human Cullin 3 (768 aa 88.9 kDa): 1 msnlskgtgs rkdtkmrira fpmtmdekyv nsiwdllkna iqeiqrknns glsfeelyrn 61 aytmvlhkhg eklytglrev vtehlinkvr edvlnslnnn flqtlnqawn dhqtamvmir 121 dilmymdrvy vqqnnvenvy nlgliifrdq vvrygcirdh lrqtlldmia rerkgevvdr 181 gairnacqml milglegrsv yeedfeapfl emsaeffqme sqkflaensa svyikkvear 241 ineeiervmh cldksteepi vkvverelis khmktiveme nsglvhmlkn gktedlgcmy 301 klfsrvpngl ktmcecmssy lreqgkalvs eegegknpvd yiqglldlks rfdrfllesf 361 nndrlfkqti agdfeyflnl nsrspeylsl fiddklkkgv kglteqevet ildkamvlfr 421 fmqekdvfer yykqhlarrl ltnksvsdds eknmisklkt ecgcqftskl egmfrdmsis 481 nttmdefrqh lqatgvslgg vdltvrvltt gywptqsatp kcnippaprh afeifrrfyl 541 akhsgrqltl qhhmgsadln atfygpvkke dgsevgvgga qvtgsntrkh ilqvstfqmt 601 ilmlfnnrek ytfeeiqqet diperelvra lqslacgkpt qrvltkepks keienghift 661 vndqftsklh rvkiqtvaak qgesdperke trqkvdddrk heieaaivri mksrkkmqhn 721 vlvaevtqql karflpspvv ikkrieglie reylartped rkvytyva |
| Format | State: Liquid (sterile filtered) containing 0.01% (w/v) Sodium Azide as preservative. Purification: Delipidation and defibrination. Purity: and |
| Applications | Western blot: This antibody reacts with human Cul3 (1/500-1/1,000; An 88.9 kDa band corresponding to human Cul3 is detected; Most cell lines expressing Cul3 can be used as a positive control).
Immunoprecipitation: The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). ELISA (1/2,000-1/10,000). |
| Description | AASequence: Human Cullin 3 (768 aa 88.9 kDa) 1 msnlskgtgs rkdtkmrira fpmtmdekyv nsiwdllkna iqeiqrknns glsfeelyrn 61 aytmvlhkhg eklytglrev vtehlinkvr edvlnslnnn flqtlnqawn dhqtamvmir 121 dilmymdrvy vqqnnvenvy nlgliifrdq vvrygcirdh lrqtlldmia rerkgevvdr 181 gairnacqml milglegrsv yeedfeapfl emsaeffqme sqkflaensa svyikkvear 241 ineeiervmh cldksteepi vkvverelis khmktiveme nsglvhmlkn gktedlgcmy 301 klfsrvpngl ktmcecmssy lreqgkalvs eegegknpvd yiqglldlks rfdrfllesf 361 nndrlfkqti agdfeyflnl nsrspeylsl fiddklkkgv kglteqevet ildkamvlfr 421 fmqekdvfer yykqhlarrl ltnksvsdds eknmisklkt ecgcqftskl egmfrdmsis 481 nttmdefrqh lqatgvslgg vdltvrvltt gywptqsatp kcnippaprh afeifrrfyl 541 akhsgrqltl qhhmgsadln atfygpvkke dgsevgvgga qvtgsntrkh ilqvstfqmt 601 ilmlfnnrek ytfeeiqqet diperelvra lqslacgkpt qrvltkepks keienghift 661 vndqftsklh rvkiqtvaak qgesdperke trqkvdddrk heieaaivri mksrkkmqhn 721 vlvaevtqql karflpspvv ikkrieglie reylartped rkvytyva Specific |
| Specificity | This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
This product reacts with human Cullin 2. Cross reactivity with Cul2 from other sources has not been determined. |
| Storage | Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Furukawa, M., He, Y.J., Borchers, C., and Xiong, Y. (2003) Targeting of ubiquitination by BTB-Cullin3-Roc1 ubiquitin ligases. Nat Cell Biol. 5, 1001-1007.
2. Kipreos,E.T., Lander,L.E., Wing,J.P., He,W.W. and Hedgecock,E.M. (1996) cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family. Cell 85 (6), 829-839. 3. Michel,J.J. and Xiong,Y. (1998) Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9 (6), 435-449. 4. Ohta,T., Michel,J.J., Schottelius,A.J. and Xiong,Y. (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol. Cell 3 (4), 535-541. 5. Mathias, N., Johnson, S.L., Winey, M., Adams, A.E., Goetsch, L., Pringle, J.R., Byers, B., Goebl, M.G. (1996) Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins. Mol Cell Biol 16:12, 6634-6643. 6. Singer,J.D., Gurian-West,M., Clurman,B. and Roberts,J.M. (1999) Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells. Genes Dev. 13 (18), 2375-2387. 7. Du,M., Sansores-Garcia,L., Zu,Z. and Wu,K.K. (1998) Cloning and expression analysis of a novel salicylate suppressible gene, Hs-CUL-3, a member of cullin/Cdc53 family. J. Biol. Chem. 273 (38), 24289-24292. 8.Jentsch S, Pyrowolakis G. (2000) Ubiquitin and its kin: how close are the family ties. Trends Cell Biol. 10(8):335-42. |
| Pictures | Figure 1. Conjugation pathways for ubiquitin and
ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin- 2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references above. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / 170,00 € | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / 160,00 € | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / 160,00 € | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / 170,00 € | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / 170,00 € | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / 200,00 € | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'R1511 Cullin-3 (CUL-3) (N-term.)'.
