![Western blot of GFP recombinant protein detected with monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. Anti-GFP detects a 27 kDa band corresponding to the epitope tag GFP. The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1.0 μg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDye® 800 conjugated Goat-a-Mouse IgG [H&L]IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](/media/images/0094356.jpeg)
R1461P GFP antibody
see related secondary antibodiessee all 73 GFP products
1 mg / US$ 575
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti GFP -
Synonyms
Green Fluorescent Protein, ,
Product review
Please read our product review about GFP: Antibody Panel to Green Fluorescent Protein (GFP).
Product Description
Mouse anti GFP -, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG2a |
| Catalog Number | R1461P |
| Swiss Prot. No. | P42212 |
| Quantity | 1 mg |
| Price | US$ 575 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1461P.pdf |
Datasheet Extract
| Monoclonal Antibody to GFP//(Green Fluorescent Protein) | |
| Concentration | 1.9 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria
Swiss Num.: P42212 |
| Format | State: Liquid (sterile filtered) purified Ig fraction Purification: Protein A affinity chromatography BufferSystem: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 0.01% sodium azide |
| Applications | This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a polyclonal anti-GFP (R1091P) using either form of the antibody as the capture or detection antibodies. Only use the monoclonal form for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (RA021HRP). While not yet tested, this antibody will likely detect GFP and GFP containing proteins by immunofluorescence microscopy, with properties similar to its companion polyclonal antibody. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on Western blots.
Recommended dilutions: ELISA 1:200,000. Western blot 1:3,000 - 1:30,000. Immunohistochemistry 1:1,000 - 1:5,000. Successful use in immunoprecipitation was reported by some researchers. As this detection method has not been verified by Acris Antibodies, the application was deleted from the database. This does not necessarily exclude the use in such procedure. |
| Specificity | Monclonal anti-GFP is designed to detect native GFP and GFP containing recombinant proteins.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). Reactivity is observed against wild type, recombinant and enhanced forms of GFP. No reaction is seen against RFP. |
| Storage | Store the antibody (in aliquots) at -20°C.
Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| Pictures | Western blot of GFP recombinant protein detected with monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. Anti-GFP detects a 27 kDa band corresponding to the epitope tag GFP. The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1.0 μg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDye® 800 conjugated Goat-a-Mouse IgG [H&L]IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1461P GFP'.