R1461B GFP antibody
see related secondary antibodiessee all 72 GFP products
1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti GFP -
Synonyms
Green Fluorescent Protein
Product review
Please read our product review about GFP: Antibody Panel to Green Fluorescent Protein (GFP).
Product Description
Mouse anti GFP -, Presentation: Biotin. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunofluorescence ( IF )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunofluorescence ( IF ) |
| Presentation | Biotin |
| Host | Mouse |
| Isotype | IgG2a |
| Catalog Number | R1461B |
| Swiss Prot. No. | P42212 |
| Quantity | 1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1461B.pdf |
Datasheet Extract
| Monoclonal Antibody to Green Fluorescent Protein (Aequorea victoria) - Biotin- | |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. Swiss Num.: P42212 |
| Format | State: Lyophilized purified Ig fraction. Purification: Protein A chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) (IgG and Protease free) as stabilizer. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molarratio: 10-20 BAC molecules per Mouse IgG molecule. Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Monclonal anti-GFP is designed to detect native GFP and GFP-containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with polyclonal anti-GFP (R1091P) using either form of the antibody as the capture or detection antibodies. Only use the monoclonal form for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (RA021HRP). While not yet tested, this antibody will likely detect GFP and GFP-containing proteins by Immunofluorescence microscopy, with properties similar to its companion polyclonal antibody. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For Immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP-containing proteins on Western blots.
Recommended dilutions: ELISA: 1:20,000-1:100,000 Western blot: 1:2,000-1:10,000 Immunohistochemistry: 1:1,000-1:5,000. |
| Specificity | This product was prepared from tissue culture supernatant by Protein A chromatography.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Mouse Serum. Reactivity is observed against wild type and recombinant forms of GFP. |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 180,00 € | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 160,00 € | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 160,00 € | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 190,00 € | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 200,00 € | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / 320,00 € | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / 320,00 € | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / 320,00 € | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / 320,00 € | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / 320,00 € | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / 330,00 € | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / 330,00 € | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'R1461B GFP'.
