R1392F Goat IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins secondary antibody
0.5 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Goat IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins -
Product Description
Rabbit anti Goat IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins -, Presentation: FITC
Properties
| Presentation | FITC |
| Host | Rabbit |
| Catalog Number | R1392F |
| Quantity | 0.5 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1392F.pdf |
Datasheet Extract
| F(ab')2 Fragment of //Rabbit anti-Goat IgG (H&L) -FITC - | |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Goat IgG whole molecule. |
| Format | State: Lyophilized F(ab')2 fragments. Purification: Immunoaffinity chromatography. BufferSystem: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide , 0.01% (w/v) Thimerosal as preservatives. Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons). Absorptionemission: 495 nm / 528 nm Molarratio: 3.3 moles FITC per mole of Rabbit IgG F(ab')2. Reconstitution: Restore with 0.5 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilution(s): 1:50 to 1:200 for most applications. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins. |
| Storage | Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 4°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| References | 1. The and Feltkamp, Immunology 18; 865, 1970.
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