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R1392AP  Goat IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins secondary antibody


see all 7 Goat IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins products
0.5 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Goat IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins -

Product Description

Rabbit anti Goat IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins -

Properties

PresentationAP
HostRabbit
Catalog NumberR1392AP
Quantity0.5 mg
Priceplease contact our distributor
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1392AP.pdf

Datasheet Extract

F(ab')2 Fragment of//Rabbit anti-Goat IgG [H&L] -AP-
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenGoat IgG whole molecule.
Format
State: Lyophilized F(ab')2 fragments.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.01M Tris Chloride, 0.25M Sodium Chloride; pH 8.0 with 15 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.05% (w/v) sodium azide as preservative.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
Reconstitution: Restore with 0.5 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA: 1:10,000 - 1:40,000.
Western Blot: 1:500 - 1:5,000.
Immunohistochemistry: 1:200 - 1:2,000.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Goat IgG and Goat Serum.
No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
StorageStore the antibody as an undiluted liquid at 4°C before opening.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result
in a substantial loss of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
References1. Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.