R1387F Goat IgG (H&L) Fab Fragment secondary antibody
1 mg / US$ 305
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Donkey anti Goat IgG (H&L) Fab Fragment -
Product Description
Donkey anti Goat IgG (H&L) Fab Fragment -, Presentation: FITC
Properties
| Presentation | FITC |
| Host | Donkey |
| Catalog Number | R1387F |
| Quantity | 1 mg |
| Price | US$ 305 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1387F.pdf |
Datasheet Extract
| Fab Fragment of //Donkey anti-Goat IgG (H&L) -FITC- | |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Goat IgG whole molecule.
|
| Format | State: Lyophilized Fab fragments Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free, and 0.01% (w/v) Sodium Azide as preservative. Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons). Absorptionemission: 495 nm / 528 nm Molarratio: 1.7 moles FITC per mole of Donkey IgG Fab. Reconstitution: Restore with 1.0 ml of deionized water or equivalent. |
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions: Flow cytometry 1:2,000 - 1:10,000 Immunofluorescence microscopy 1:500 - 1:2,500. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Donkey Serum. No reaction was observed against anti-Pepsin or anti-Donkey IgG F(c). |
| Storage | Store vial at 4°C prior to restoration. centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 4°C as an undiluted liquid.
For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | 1. The and Feltkamp, Immunology 18; 865, 1970. (Conjugation) |
