R1364B Rabbit IgG (H&L) secondary antibody
see all 27 Rabbit IgG (H&L) products
2 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Rabbit IgG (H&L) -
Product Description
Goat anti Rabbit IgG (H&L) -, Presentation: Biotin
Properties
| Presentation | Biotin |
| Host | Goat |
| Catalog Number | R1364B |
| Quantity | 2 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1364B.pdf |
Datasheet Extract
| Polyclonal Antibody to Rabbit IgG [H&L] //-Biotin- | |
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Rabbit IgG whole molecule. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) sodium azide Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molarratio: 10-20 BAC molecules per Goat IgG molecule Reconstitution: Restore with 1.0 ml of deionized water or equivalent. |
| Applications | Immunoblotting (1/1,000-1/5,000 for Western blots).
ELISA (1/10,000-1/50,000). Immunohistochemistry (1/400-1/2,000). Immunomicroscopy as well as other assays using streptavidin/avidin conjugates requiring lot-to-lot consistency. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. |
| Storage | Store vial at 2-8°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | (Conjugation): Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. |
