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R1362HRP  Mouse IgG3 gamma 3 chain secondary antibody


see all 7 Mouse IgG3 gamma 3 chain products
1 mg / US$ 385
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Mouse IgG3 gamma 3 chain

Product Description

Rabbit anti Mouse IgG3 gamma 3 chain , Presentation: HRP

Properties

PresentationHRP
HostRabbit
Catalog NumberR1362HRP
Quantity1 mg
PriceUS$ 385
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1362HRP.pdf

Datasheet Extract

Alternate namesMouse Immunoglobulin gamma 3 chain specific.
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenMouse IgG3 γ3 chain.
Format
State: Lyophilized purified Ig fraction.
Purification: Prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative
Label: Horseradish Peroxidase (HRP)
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for immunoblotting (Western or dot blot: 1/1,000-5,000), ELISA (1/5,000-1/20,000), immunoperoxidase electron microscopy and immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Species: Mouse.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
References(Conjugation) Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981