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R1362B  Mouse IgG3 gamma 3 chain secondary antibody


see all 7 Mouse IgG3 gamma 3 chain products
1 mg / US$ 365
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Mouse IgG3 gamma 3 chain

Product Description

Rabbit anti Mouse IgG3 gamma 3 chain , Presentation: Biotin

Properties

PresentationBiotin
HostRabbit
Catalog NumberR1362B
Quantity1 mg
PriceUS$ 365
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1362B.pdf

Datasheet Extract

Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenMouse IgG3 heavy chain.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
Molarratio: 10-20 BAC molecules per Rabbit IgG molecule.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/8,000-1/32,000.
Western Blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500- 1/2,000.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit was confirmed by ELISA.
Typically less than 1% cross reactivity was observed against other Mouse heavy chain isotypes.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
References(Conjugation) Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990.