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R1361F  Mouse IgG2b gamma 2b chain secondary antibody


see all 7 Mouse IgG2b gamma 2b chain products
1 mg / US$ 355
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Mouse IgG2b gamma 2b chain

Product Description

Rabbit anti Mouse IgG2b gamma 2b chain , Presentation: FITC

Properties

PresentationFITC
HostRabbit
Catalog NumberR1361F
Quantity1 mg
PriceUS$ 355
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1361F.pdf

Datasheet Extract

Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenMouse IgG2b γ2b chain.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA) (IgG and Protease free) as stabilizer.
Label: Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons).
Absorptionemission: 495 nm / 528 nm
Molarratio: 2.4 moles FITC per mole of Rabbit IgG.
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended dilutions:
FACS: 1/2,000-1/10,000.
IF microscopy: 1/500-1/2,500.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum and Mouse Serum.
Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
References(Conjugation) The and Feltkamp, Immunology 18; 865, 1970.