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R1331AP  Equine IgG F(ab')2 secondary antibody

1 mg / US$ 375
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Equine IgG F(ab')2 -

Product Description

Rabbit anti Equine IgG F(ab')2 -

Properties

PresentationAP
HostRabbit
Catalog NumberR1331AP
Quantity1 mg
PriceUS$ 375
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1331AP.pdf

Datasheet Extract

Polyclonal Antibody to Horse IgG F(ab')2 -AP-
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenHorse IgG F(ab')2 fragment.
Format
State: Liquid (sterile filtered) purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons).
ApplicationsSuitable for Immunoblotting (Western blot: 1/500-1/2,000 or Dot blot), ELISA (1/2,000-1/8,000) and Immunohistochemistry (1/200-1/1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Horse IgG, Horse IgG F(ab') 2 and Horse Serum.
No reaction was observed against Horse IgG F(c).
StorageStore the antibody at 2-8°C as an undiluted liquid.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss
of enzymatic activity.
Dilute only prior to immediate use.
Shelf life: One year from despatch.
ReferencesModified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. (Conjugation)