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R1329HRP  Equine IgG (H&L) secondary antibody


see all 24 Equine IgG products
2 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Equine IgG (H&L) -

Product Description

Rabbit anti Equine IgG (H&L) -, Presentation: HRP

Properties

PresentationHRP
HostRabbit
Catalog NumberR1329HRP
Quantity2 mg
Priceplease contact our distributor
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1329HRP.pdf

Datasheet Extract

Polyclonal Antibody to Horse IgG [H&L] -HRP-
Concentration2.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenHorse IgG whole molecule.
Format
State: Lyophilized purified Ig fraction.
Purification: Immunoaffinity chromatography.
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
Label: Horseradish Peroxidase (HRP).
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsSuitable for Immunoblotting (Western blot: 1/5,000-1/20,000 or Dot blot), ELISA (1/50,000-1/200,000), Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Horse IgG and Horse Serum.
StorageStore vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
CautionDo Not Use Sodium Azide as preservative.
ReferencesFarr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)