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R1318HRP  Goat IgG (H&L) ads. to Hu serum Proteins secondary antibody

1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Goat IgG (H&L) ads. to Hu serum Proteins -

Product Description

Rabbit anti Goat IgG (H&L) ads. to Hu serum Proteins -, Presentation: HRP

Properties

PresentationHRP
HostRabbit
Catalog NumberR1318HRP
Quantity1 mg
Priceplease contact our distributor
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1318HRP.pdf

Datasheet Extract

Polyclonal Antibody to Goat IgG (H&L) - HRP
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenGoat IgG whole molecule

Format
State: Lyophilized purified Ig fraction
Purification: Immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Purity: and
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.

Label: Horseradish Peroxidase (HRP)
Reconstitution: Restore with 1.0 ml deionized water or equivalent.

ApplicationsSuitable for immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA 1:14,000 - 1:60,000
Western blot 1:1,000 - 1:5,000
Immunohistochemistry 1:500 - 1:2,500
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum.
No reaction was observed against Human Serum Proteins.

StorageStore vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.

ReferencesFarr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)