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R1253T  Mouse IgG (H&L) secondary antibody


see all 154 Mouse IgG products
2 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Rabbit anti Mouse IgG (H&L) -

Product Description

Rabbit anti Mouse IgG (H&L) -, Presentation: TRITC

Properties

PresentationTRITC
HostRabbit
Catalog NumberR1253T
Quantity2 mg
Priceplease contact our distributor
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1253T.pdf

Datasheet Extract

Polyclonal Antibody to Mouse IgG [H&L] - TRITC
Concentration2.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenMouse IgG whole molecule

Format
State: Lyophilzed IgG fraction
Purification: Immunoaffinity chromatography
Purity: and
BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide
Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)
Absorptionemission: 550 nm / 570 nm
Molarratio: 3.0 moles TRITC per mole of Rabbit IgG
Reconstitution: Restore with 1.0 ml of deionized water or equivalent.
ApplicationsSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended dilution(s):
Flow cytometry 1:2,000 - 1:10,000.
Immunofluorescence microscopy 1:500 - 1:2,500.
SpecificityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.
StorageStore vial at 2-8°C prior to restoration.
Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
Dilute only prior to immediate use.
Shelf life: one year from despatch.
References(Conjugation) J.A. Titus, et al. (1982) J. Immunol. Methods 50: 193.