R1253HRP Mouse IgG (H&L) secondary antibody
see all 272 Mouse IgG products
2 mg / US$ 325
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Mouse IgG (H&L)
Product Description
Rabbit anti Mouse IgG (H&L) , Presentation: HRP
Properties
| Presentation | HRP |
| Host | Rabbit |
| Catalog Number | R1253HRP |
| Quantity | 2 mg |
| Price | US$ 325 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1253HRP.pdf |
Datasheet Extract
| Purified Rabbit anti-Mouse IgG [H&L] - HRP | |
| Concentration | 2.0 mg/ml (by UV absorbance at 280nm) |
| Immunogen | Mouse IgG whole molecule |
| Format | State: Lyophilized Ig fraction Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Purity: and BufferSystem: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer Label: Horseradish Peroxidase (HRP) Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions ELISA: 1:540,000. WESTERN BLOT: 1:2,000 - 1:10,000. IMMUNOHISTOCHEMISTRY: 1:1,000 - 1:5,000. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. |
| Storage | Store vial at 2-8° C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2-8° C as an undiluted liquid.
For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use. Shelf life: one year from despatch. |
| References | Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. |