R1253B Mouse IgG (H&L) secondary antibody
see all 154 Mouse IgG products
2 mg / US$ 305
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Mouse IgG (H&L) -
Product Description
Rabbit anti Mouse IgG (H&L) -, Presentation: Biotin
Properties
| Presentation | Biotin |
| Host | Rabbit |
| Catalog Number | R1253B |
| Quantity | 2 mg |
| Price | US$ 305 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1253B.pdf |
Datasheet Extract
| Polyclonal Antibody to Mouse IgG [H&L] - Biotin | |
| Concentration | 2.0 mg/ml (by UV absorbance at 280nm) |
| Immunogen | Mouse IgG whole molecule
|
| Format | State: Lyohilized Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Purity: and BufferSystem: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4, containing 10 mg/ml BSA, IgG and Protease free, as stabilizer and 0.01% (w/v) Thimerisol as preservative Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molarratio: 10-20 BAC molecules per Rabbit IgG molecule Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin (code RA021HRP) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:90,000 of the reconstitution concentration is suggested for this product. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
|
| Storage | Store vial at 2 - 8 °C prior to restoration.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 2 - 8 ° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| References | Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation) |