R1251B Donkey IgG (H&L) secondary antibody
see all 21 Donkey IgG products
2 mg / US$ 305
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Rabbit anti Donkey IgG (H&L)
Product Description
Rabbit anti Donkey IgG (H&L) , Presentation: Biotin
Properties
| Presentation | Biotin |
| Host | Rabbit |
| Catalog Number | R1251B |
| Quantity | 2 mg |
| Price | US$ 305 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1251B.pdf |
Datasheet Extract
| Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Donkey IgG whole molecules. |
| Format | State: Lyophilized purified Ig fraction. Purification: Immunoaffinity chromatography. Purity: and BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% sodium azide as a preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Molarratio: 10-20 BAC molecules per Goat IgG molecule. Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Donkey IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:45,000 to 1:150,000 of the reconstitution concentration is suggested for this product. |
| Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Donkey IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Donkey IgG and Donkey Serum. |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. (Conjugation)
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