R1094P Lactate Dehydrogenase (LDH) antibody
see related secondary antibodiessee all 22 Lactate Dehydrogenase products
50 mg / US$ 465
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti Lactate Dehydrogenase (LDH) -
Product Description
Goat anti Lactate Dehydrogenase (LDH) -, Presentation: Ig Fraction. Product is tested for Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Reactivity | Rabbit ( Rb ) |
| Presentation | Ig Fraction |
| Host | Goat |
| Catalog Number | R1094P |
| Swiss Prot. No. | P13491 |
| Quantity | 50 mg |
| Price | US$ 465 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1094P.pdf |
Datasheet Extract
| Polyclonal Antiobody to //Lactate Dehydrogenase [Rabbit Muscle] | |
| Alternate names | L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit |
| Concentration | 10 mg/ml (by UV absorbance at 280 nm) |
| Background | Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36 kDa subunits. Every LDH molecule consists of four subunits, where each subunit is either H or M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H) - in the heart, LDH-2 (3H1M) - in the reticuloendothelial system, LDH-3 (2H2M) - in the lungs, LDH-4 (1H3M) - in the kidneys and LDH-5 (4M) - in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a "flipped pattern") suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.
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| Immunogen | Full length Lactate Dehydrogenase from Rabbit Muscle. Swiss Num.: P13491 AA Sequence: Protein Sequence: 332 aa, predicted MW 36.6kDa 1 maalkdqlih nllkeehvpq nkitvvgvga vgmacaisil mkdladelal vdvmedklkg 61 emmdlqhgsl flrtpkivsg kdysvtansk lviitagarq qegesrlnlv qrnvnifkfi 121 ipnvvkysph ckllvvsnpv diltyvawki sgfpknrvig sgcnldsarf rylmgerlgv 181 halschgwil gehgdssvpv wsgmnvagvs lktlhpelgt dadkeqwkqv hkqvvdsaye 241 viklkgyttw aiglsvadla esimknlrrv hpistmlkgl ygikedvfls vpcvlgqngi 301 sdvvkvtlts eeeahlkksa dtlwgiqkel qf |
| Format | State: Lyophilized purified Ig fraction. Purification: Multi-step process. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% (w/v) Sodium Azide. Reconstitution: Restore with 5.0 ml of deionized water (or equivalent). |
| Applications | Suitable for use in ELISA (1/2,000-1/10,000) and by Western blot (1/1,000-1/5,000). Expect a band approximately 36 kDa in size corresponding to LDH western blotting in the appropriate cell lysate or extract.
Recommended Dilutions: This product has been assayed against 1.0 µg of Lactate Dehydrogenase [Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and ABTS (2,2â??-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product. |
| Description | AASequence: Protein Sequence: 332 aa, predicted MW 36.6kDa 1 maalkdqlih nllkeehvpq nkitvvgvga vgmacaisil mkdladelal vdvmedklkg 61 emmdlqhgsl flrtpkivsg kdysvtansk lviitagarq qegesrlnlv qrnvnifkfi 121 ipnvvkysph ckllvvsnpv diltyvawki sgfpknrvig sgcnldsarf rylmgerlgv 181 halschgwil gehgdssvpv wsgmnvagvs lktlhpelgt dadkeqwkqv hkqvvdsaye 241 viklkgyttw aiglsvadla esimknlrrv hpistmlkgl ygikedvfls vpcvlgqngi 301 sdvvkvtlts eeeahlkksa dtlwgiqkel qf |
| Specificity | This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]. BLAST analysis was used to determine that cross reactivity is suggested for both muscle and heart isoforms (LDH-A and LDH-B) from most mammalian species. |
| Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. Shelf life: One year from despatch. |
| References | 1. Zaman K, et al. (1999) Protection from oxidative stress-induced apoptosis in cortical neuronal cultures by iron chelators is associated with enhanced DNA binding of hypoxia-inducible factor-1 and ATF-1/CREB and increased expression of glycolytic enzymes, p21(waf1/cip1), and erythropoietin. J Neurosci 19(22):9821-9830.
2. Sass,C., Briand,M., Benslimane,S., Renaud,M. and Briand,Y. (1989) Characterization of rabbit lactate dehydrogenase-M and lactate dehydrogenase-H cDNAs. Control of lactate dehydrogenase expression in rabbit muscle. J. Biol. Chem. 264 (7), 4076-4081. |
| Pictures | Figure 1. Western blot analysis shows Anti-Lactate
Dehydrogenase antibody detects LDH in HeLa cell extracts. Reactivity with LDH is observed in the cytoplasmic fraction (CF) and little to no reactivity in the nuclear fraction (NF). ~30 µg was loaded per lane onto a 10% gel for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the protein. The blot was incubated with a 1:1,000 dilution of the antibody in 5% milk in TBST at room temperature followed by detection using standard procedures. Personal communication Kuldeep Patel. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1317B | Goat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1317F | Goat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1317T | Goat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1317TR | Goat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1317HRP | Goat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 305 | |||||
| R1317AP | Goat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 315 | |||||
| R1435C2 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy2 | 1 mg / US$ 445 | |||||
| R1435C3 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy3 | 1 mg / US$ 445 | |||||
| R1435C35 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy3.5 | 1 mg / US$ 445 | |||||
| R1435C5 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy5 | 1 mg / US$ 465 | |||||
| R1435C55 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy5.5 | 1 mg / US$ 445 | |||||
| R1416R | Goat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1094P Lactate Dehydrogenase (LDH)'.