R1091TR GFP antibody
see related secondary antibodiessee all 56 GFP products
1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti GFP -
Synonyms
Green Fluorescent Protein
Product review
Please read our product review about GFP: Antibody Panel to Green Fluorescent Protein (GFP).
Product Description
Goat anti GFP -, Presentation: Texas Red. Product is tested for Frozen sections ( C ), Immunofluorescence ( IF )
Properties
| Applications | Frozen sections ( C ), Immunofluorescence ( IF ) |
| Presentation | Texas Red |
| Host | Goat |
| Catalog Number | R1091TR |
| Swiss Prot. No. | P42212 |
| Quantity | 1 mg |
| Price | please contact our distributor |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1091TR.pdf |
Datasheet Extract
| Polyclonal antibody to //Green Fluorescent Protein (GFP)// -Texas Red (TM)- | |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | The immunogen is a GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. Swiss Num.: P42212 |
| Format | State: Lyophilized purified Ig fraction. Purification: Protein A chromatography. Purity: / BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 witn 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Texas Red (TM) (TR; MW 625 daltons). Absorptionemission: 596 nm / 620 nm Molarratio: 3.0 moles FITC per mole of Goat IgG. Reconstitution: Restore with 1.0 ml of deionized water (or equivalent). |
| Applications | Designed to detect GFP and its variants in ELISA (sandwich or capture), Immunoblotting and Immunoprecipitation.
Monoclonal and polyclonal forms of anti-GFP assayed by ELISA for direct binding of antigen recognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited to titrate GFP in solution using either form of the antibody as the capture or detection antibodies. The detection antibody is typically conjugated to biotin and complexed with streptavidin-HRP. Fluorochrome conjugated anti-GFP was assayed by Immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Peroxidase conjugated anti-GFP assayed by immunoblot shows a 42 kDa band when reacted with GFP on a western blot. |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum.
Reactivity is observed against wild type, recombinant and enhanced forms of GFP. |
| Storage | Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20°C or below. This product is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: One year from despatch. |
| References | J.A. Titus, et.al. J. Immunol. Methods 50; 193, 1982. (Conjugation)
|
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1317B | Goat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / 170,00 € | |||||
| R1317F | Goat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / 170,00 € | |||||
| R1317T | Goat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / 170,00 € | |||||
| R1317TR | Goat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / 170,00 € | |||||
| R1317HRP | Goat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / 180,00 € | |||||
| R1317AP | Goat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / 190,00 € | |||||
| R1435C2 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy2 | 1 mg / 320,00 € | |||||
| R1435C3 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy3 | 1 mg / 320,00 € | |||||
| R1435C35 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy3.5 | 1 mg / 320,00 € | |||||
| R1435C5 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy5 | 1 mg / 340,00 € | |||||
| R1435C55 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy5.5 | 1 mg / 320,00 € | |||||
| R1416R | Goat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / 330,00 € | |||||
Click here to see all secondary antibodies for 'R1091TR GFP'.
