Acris Antibodies Logo

Antibodies and related products for scientific research: primary antibodies: 70.970, secondary ab: 1.096, proteins: 20.574, kits: 265, other products: 1.054

No staining picture available ...

 

Zoom Out
Zoom In

R1091T  GFP antibody

see related secondary antibodies
see all 72 GFP products
1 mg / please contact our distributor
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com

Quick Overview

Goat anti GFP -

Synonyms

Green Fluorescent Protein

Product review
Please read our product review about GFP: Antibody Panel to Green Fluorescent Protein (GFP).

Product Description

Goat anti GFP -, Presentation: TRITC. Product is tested for Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )

Properties

ApplicationsEnzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Westernblot / Immunoblot ( WB )
PresentationTRITC
HostGoat
Catalog NumberR1091T
Swiss Prot. No.P42212
Quantity1 mg
Priceplease contact our distributor
DeliveryWorldwide
ManufacturerAcris Antibodies GmbH
Datasheetview PDF-Download
R1091T.pdf

Datasheet Extract

Polyclonal Antibody to Green Fluorescent Protein (GFP) - TRITC
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
ImmunogenGST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria
Swiss Num.: P42212
Format
State: Lyophilized Ig fraction
Purification: Immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Purity: /
BufferSystem: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 as a buffer, 10 mg/ml Bovine Serum Albumin (BSA) IgG and (protease free) as stabilizer, 0.01 % (w/v) Sodium azide as preservative
Label: TRITC (Molecular weight 444 daltons)
Absorptionemission: 550 nm / 570 nm
Molarratio: 2.9 moles TRITC per mole of Goat IgG
Reconstitution: Restore with 1.0 ml of deionized water (or equivalent).
ApplicationsElisa (sandwich or capture; ELISA for direct binding recognizes wild type, recombinant and enhanced forms of GFP; for sandwich ELISA titrate GFP in solution using either form of the antibody as the capture or detection antibodies, detection antibody is typically conjugated to to biotin and complexed with streptavidin-HRP).
Western blot (Peroxidase conjugated anti-GFP shows a 42 kDa band).
Immunoprecipitation.
Immunoflourescence.
SpecificityThis antibody detects Fluorescent Protein (GFP).
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
StorageStore vial at 4° C prior to reconstitution.
Following reconstitution the product is stable for one month at 2 - 8 °C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage add glycerol to 50 % and then aliquot contents and freeze at -20 °C or below. Avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
Shelf life: One year from despatch.

12 Secondary Antibodies

Catalog No.Name / HostPresentationReactivity 
R1317B Goat IgG (H&L)  
  Rabbit Biotin   2 mg / 170,00 €
   
R1317F Goat IgG (H&L)  
  Rabbit FITC   2 mg / 170,00 €
   
R1317T Goat IgG (H&L)  
  Rabbit TRITC   2 mg / 170,00 €
   
R1317TR Goat IgG (H&L)  
  Rabbit Texas Red   2 mg / 170,00 €
   
R1317HRP Goat IgG (H&L)  
  Rabbit HRP   2 mg / 180,00 €
   
R1317AP Goat IgG (H&L)  
  Rabbit AP   1 mg / 190,00 €
   
R1435C2 Goat IgG (H&L) multi-species ads.  
  Donkey Cy2   1 mg / 320,00 €
   
R1435C3 Goat IgG (H&L) multi-species ads.  
  Donkey Cy3   1 mg / 320,00 €
   
R1435C35 Goat IgG (H&L) multi-species ads.  
  Donkey Cy3.5   1 mg / 320,00 €
   
R1435C5 Goat IgG (H&L) multi-species ads.  
  Donkey Cy5   1 mg / 340,00 €
   
R1435C55 Goat IgG (H&L) multi-species ads.  
  Donkey Cy5.5   1 mg / 320,00 €
   
R1416R Goat IgG (H&L) F(ab')2 Fragment multi-species ads.  
  Donkey PE   1 ml / 330,00 €
   

Click here to see all secondary antibodies for 'R1091T GFP'.