![Western blot of GFP recombinant protein
detected with polyclonal anti-GFP antibody. Lane 1 shows blot results where GFP
recombinant protein was expressed in HeLa cells.
Lane 2 shows control staining of HeLa lysate not
expressing GFP. Polyclonal anti-GFP detects a 33 kDa band corresponding to the epitope tag GFP. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After
blocking the membrane was probed with the primary
antibody diluted to 1.0 μg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDye(R) 800 conjugated Donkey-a-
Goat IgG [H&L] . IRDye(R)800
fluorescence image was captured using the Odyssey(R)
Infrared Imaging System developed by LI-COR. IRDye
is a trademark of LI-COR, Inc. Other detection
systems will yield similar results.](/media/images/0093946.jpeg)
R1091P GFP antibody
see related secondary antibodiessee all 73 GFP products
1 mg / US$ 485
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Goat anti GFP -
Synonyms
Green Fluorescent Protein, ,
Product review
Please read our product review about GFP: Antibody Panel to Green Fluorescent Protein (GFP).
Product Description
Goat anti GFP -, Presentation: Ig Fraction. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Presentation | Ig Fraction |
| Host | Goat |
| Catalog Number | R1091P |
| Swiss Prot. No. | P42212 |
| Quantity | 1 mg |
| Price | US$ 485 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  R1091P.pdf |
Datasheet Extract
| Polyclonal Antibody to //Green Fluorescent Protein (GFP) | |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Immunogen | Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria Swiss Num.: P42212 |
| Format | State: Liquid, sterile filtered antibody Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative |
| Applications | Polyclonal anti-GFP is designed to detect GFP and its variants. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen and recognizes wild type, recombinant and enhanced forms of GFP.
Biotin conjugated polyclonal anti-GFP used in a sandwich ELISA is well suited to titrate GFP in solution using this antibody in combination with a monoclonal anti-GFP (R1461P) using either form of the antibody as the capture or detection antibodies. However, use the monoclonal form only for the detection of wild type or recombinant GFP as this form does not sufficiently detect 'enhanced' GFP. The detection antibody is typically conjugated to biotin and subsequently reacted with streptavidin conjugated HRP (RA021HRP). Fluorochrome conjugated polyclonal anti-GFP can be used to detect GFP by immunofluorescence microscopy in prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and can detect GFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-GFP relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-GFP to detect GFP or GFP containing proteins on western blots. Recommended dilutions: ELISA (1:40,000). Western blot (1:400 - 1:2,000). Immunohistochemistry (1:200 - 1:1,000). |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria) serum.
No reaction was observed against Human, Mouse or Rat serum proteins. |
| Storage | Store vial at -20°C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Shelf life: one year from despatch. |
| Pictures | Western blot of GFP recombinant protein
detected with polyclonal anti-GFP antibody. Lane 1 shows blot results where GFP recombinant protein was expressed in HeLa cells. Lane 2 shows control staining of HeLa lysate not expressing GFP. Polyclonal anti-GFP detects a 33 kDa band corresponding to the epitope tag GFP. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted to 1.0 μg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDye(R) 800 conjugated Donkey-a- Goat IgG [H&L] . IRDye(R)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1317B | Goat IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 295 | |||||
| R1317F | Goat IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 295 | |||||
| R1317T | Goat IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 295 | |||||
| R1317TR | Goat IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1317HRP | Goat IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 305 | |||||
| R1317AP | Goat IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 315 | |||||
| R1435C2 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy2 | 1 mg / US$ 445 | |||||
| R1435C3 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy3 | 1 mg / US$ 445 | |||||
| R1435C35 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy3.5 | 1 mg / US$ 445 | |||||
| R1435C5 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy5 | 1 mg / US$ 465 | |||||
| R1435C55 | Goat IgG (H&L) multi-species ads. | ||||||
| Donkey | Cy5.5 | 1 mg / US$ 445 | |||||
| R1416R | Goat IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'R1091P GFP'.