PA212X Bone Morphogenetic Protein 2 (BMP2) antigen
see all 18 Bone Morphogenetic Protein 2 products
10 µg / US$ 355
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Bone Morphogenetic Protein 2 (BMP2)
Synonyms
BMP-2,
Product Description
Human Bone Morphogenetic Protein 2 (BMP2)
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >98% |
| Source | E. coli |
| Catalog Number | PA212X |
| Quantity | 10 µg |
| Price | US$ 355 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA212X.pdf |
Datasheet Extract
| Alternate names | BMP-2A
|
| Background | BMPs (Bone Morphogenetic Proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. |
| Source | E.coli |
| Format | State: Lyophilized purified fraction (>98% pure by SDS-PAGE). BufferSystem: 5 mM Sodium Citrate, pH 3.0 without preservatives. Reconstitution: Restore in water containing BSA (50 µg of BSA per 1 µg of protein) to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use |
| Description | Recombinant human BMP-2 is a homodimeric protein consisting of two 115 amino acid polypeptide chains. AASequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR. Biological Activity: The ED50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 cells is < 1 µg/ml. Molecular weight: 26 kDa |
| Add. Information | Centrifuge the vial prior to opening! |
| Storage | Store the lyophilized protein at -20°C.
Reconstituted BMP-2 should be stored in working aliquots with a carrier protein at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Lyssiotis, C.A. Inhibition of histone deacetylase activity induces developmental plasticity in oligodendrocyte precursor cells. Proceedings of the National Academy of Sciences of the U S A., 2007, 104(38):14982-7.
2. Chung, Y.I. Enhanced bone regeneration with BMP-2 loaded functional nanoparticle-hydrogel complex. Journal of Controlled Release, 2007, 121(1-2):91-9. 3. Huang, M.S. Atherogenic Phospholipids Attenuate Osteogenic Signaling by BMP-2 and Parathyroid Hormone in Osteoblasts. The Journal of Biological Chemistry, 2007, 282(29):21237-21243. 4. Giuliani N, The proteasome inhibitor bortezomib affects osteoblast differentiation in vitro and in vivo in multiple myeloma patients Blood, 2007, 110(1):334-8. 5. Wong, C.E. Neural crest-derived cells with stem cell features can be traced back to multiple lineages in the adult skin. Journal of Cell Biology, 2006, 175(6):1005-15. 6. Chou YT Post-transcriptional Control of Cited2 by Transforming Growth Factor beta: REGULATION VIA SMADS AND CITED2 CODING REGION. J Biol Chem., 2006, 281(27):18451-62. 7. Yamashita A Osteoblastic differentiation of monkey embryonic stem cells in vitro Cloning Stem Cells, 2005, 7(4):232-7. 8. Giuliani N Myeloma cells block RUNX2/CBFA1 activity in human bone marrow osteoblast progenitors and inhibit osteoblast formation and differentiation Blood, 2005, 106(7):2472-83. |
