PA1234 MAP kinase p38 alpha / MAPK14 antigen
see all 36 MAP kinase p38 alpha / MAPK14 products
10 µg / US$ 225
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human MAP kinase p38 alpha / MAPK14
Synonyms
CSAID-binding protein, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, EXIP, p38ALPHA, , Mitogen-activated protein kinase 14, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2
Product review
Please read our product review about MAP kinase p38 alpha / MAPK14: Antibodies to MAP-Kinases.
Product Description
Human MAP kinase p38 alpha / MAPK14
Properties
| Presentation | Purified |
| Species | Human |
| Purity | >90% |
| Source | E. coli |
| Molecular Weight | 40 kD |
| Catalog Number | PA1234 |
| Swiss Prot. No. | Q16539 |
| Quantity | 10 µg |
| Price | US$ 225 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA1234.pdf |
Datasheet Extract
| Alternate names | Protein phosphatase 1A, EC 3.1.3.16, Protein phosphatase 2C isoform alpha, PP2C-alpha, IA, PPM1A, PP2CA, MGC9201 |
| Concentration | 1,0 mg/ml |
| Background | Protein Phosphatase 2C alpha is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding two distinct isoforms have been described.
Protein phosphatase 2C(PP2Cα) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. |
| Immunogen | Swiss Num.: Q16539 |
| Source | Escherichia Coli |
| Format | State: Liquid purified protein (Sterile filtered colorless solution) Purity: Greater than 95.0% as determined by: (a)Analysis by RP-HPLC. (b)Analysis by SDS-PAGE. BufferSystem: 10mM Tris-HCl, pH7.5, 50mM NaCl, 2mM DTT, 1mM MnCl2 and 20% glycerol |
| Description | Protein Phosphatase 2C alpha Human Recombinant produced is a single,non-glycosylated polypeptide chain conjtaining 382 amino acids (containing His tag, T7 gene 10 leader, XpressTM Epitope). The protein coding region of PP2Cα (amino acids 1-382) was cloned into an E. coli expression vector(BamHI/HindШ site). PP2Cα was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.
AASequence: MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWILMGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW Specific Activity: 8,000 U/mg (One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37°C using 10mM of substrate) Molecular weight: 46.6KDa |
| Storage | Store the protein undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Shelf life: one year from despatch. |
