PA102 Macrophage Colony Stimulating Factor (M-CSF) antigen
see all 76 Macrophage Colony Stimulating Factor products
2 µg / US$ 255
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Human Macrophage Colony Stimulating Factor (M-CSF)
Synonyms
MCSF
Product Description
Human Macrophage Colony Stimulating Factor (M-CSF) ;Is tested for Flow cytometry (F)
Properties
| Species | Human |
| Purity | >98% |
| Source | E. coli |
| Molecular Weight | 18.4 kD |
| Catalog Number | PA102 |
| Quantity | 2 µg |
| Price | US$ 255 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  PA102.pdf |
Datasheet Extract
| Recombinant Human M-CSF | |
| Alternate names | Macrophage Colony Stimulating Factor, CSF-1, MGI-IM
|
| Background | M-CSF is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. |
| Source | E.coli |
| Format | State: Sterile filtered, lyophilized. Purity: > 98% by SDS-PAGE and HPLC analyses BufferSystem: Sodium phosphate, pH 8.0, + 75 mM NaCl Endotoxinlevel: < 0.1 ng per µg (1EU/µg) Reconstitution: Centrifuge the vial prior to opening. Restore in water to a concentration of 0.1-0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 2-8°C for 1 week or -20°C for future use. |
| Description | Recombinant human M-CSF is a 36.8 kDa homodimeric protein containing two 159 amino acid polypeptide subunits. AASequence: MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NSF-60 cells is < / = 5.0 ng/ml. Specific Activity: >/= 2 x 10e5 units/mg Molecular weight: 18.4 kDa |
| Storage | The lyophilized protein is stable at -20°C for one year from date of despatch.
Reconstituted M-CSF is stable for 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freezing and thawing. |
| References | 1. Senju S, Genetically manipulated human embryonic stem cell-derived dendritic cells with immune regulatory function Stem Cells, 2007, 25(11):2720-9.
2. Szatmari I, PPARgamma regulates the function of human dendritic cells primarily by altering lipid metabolism Blood, 2007, 110(9):3271-80. 3. Li G, TGF-beta combined with M-CSF and IL-4 induces generation of immune inhibitory cord blood dendritic cells capable of enhancing cytokine-induced ex vivo expansion of myeloid progenitors Blood, 2007, 110(8):2872-9. 4. Tai YT, Targeting MEK induces myeloma-cell cytotoxicity and inhibits osteoclastogenesis Blood, 2007, 110(5):1656-63. 5. Park, H. The Yersinia effector protein YpkA induces apoptosis independently of actin depolymerization. The Journal of Immunology, 2007, 178(10):6426-34. |
