NB800-PC23 Normal Rat Liver Whole Cell Extract (Positive Control)
0.15 ml / US$ 295 no delivery possible
Quick Overview
Normal Rat Liver Whole Cell Extract (Positive Control)
Synonyms
Lysates,
Product Description
Normal Rat Liver Whole Cell Extract (Positive Control)
Properties
| Catalog Number | NB800-PC23 |
| Quantity | 0.15 ml |
| Price | US$ 295 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet | view  NB800-PC23.pdf |
Datasheet Extract
| Normal Rat Liver Whole Cell Extract | |
| Concentration | 1 mg/ml |
| Background | This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins. |
| Format | BufferSystem: 1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8] |
| Applications | Western Blot |
| Add. Information | Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95 degrees C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 0.01-0.02 ml depending on the size format of your gel. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
