NB600-601 GFP antibody
see related secondary antibodiessee all 73 GFP products
0.5 mg / no delivery possible
Quick Overview
Rabbit anti GFP -
Synonyms
Green Fluorescent Protein, ,
Product review
Please read our product review about GFP: Antibody Panel to Green Fluorescent Protein (GFP).
Product Description
Rabbit anti GFP -, Presentation: Aff - Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | NB600-601 |
| Swiss Prot. No. | P42212 |
| Quantity | 0.5 mg |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet | view  NB600-601.pdf |
Datasheet Extract
| Rabbit Polyclonal anti-GFP | |
| Alternate names | anti-Green Fluorescent Protein antibody |
| Concentration | 1.0 mg/ml |
| Background | Green fluorescence protein (GFP) is a 27 KDa protein derived from the jellyfish Aquorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. |
| Immunogen | GFP fusion protein to the full-length protein derived from the jellyfish Aequorea victoria. NB 600-601 was prepared from monospecific antiserum by immunoaffinity chromatography using GFP (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove unwanted reactivities. Swiss Num.: P42212 |
| Format | Purification: Affinity purified Purity: Affinity purified BufferSystem: 0.02M KPO4, 0.15 M NaCl [pH 7.2] |
| Applications | ELISA 1:10,000 - 1:50,000, immunohistochemistry 1:500 - 1:3,000, Western Blot 1:500 - 1:5,000 |
| Specificity | Species: Reactive against all variants of (Aequorea victoria) GFP such as S65T-GFP, RS-GFP, YFP and EGFP. |
| Storage | Store at 4C for short term. Aliquot and store at -20C for long term. Avoid freeze thaw cycles. |
| References | 1. Zhang QX et al. Identification of structurally important domains of lipid phosphate phosphatase-1: implications for its sites of action. Biochem J 345 Pt 2:181-4 (2000). PubMed: 10620492
2. Mesaeli N et al. Calreticulin is essential for cardiac development. J Cell Biol 144:857-68 (1999). PubMed: 10085286 3. McCabe JB & Berthiaume LG Functional roles for fatty acylated amino-terminal domains in subcellular localization. Mol Biol Cell 10:3771-86 (1999). PubMed: 10564270 4. Jasinska R et al. Lipid phosphate phosphohydrolase-1 degrades exogenous glycerolipid and sphingolipid phosphate esters. Biochem J 340 ( Pt 3):677-86 (1999). PubMed: 10359651 |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB600-601 GFP'.