NB600-567 Tubulin (acetyl K40) antibody
see related secondary antibodiessee all 213 Tubulin products
0.1 ml / US$ 445 no delivery possible
Quick Overview
Mouse anti Tubulin (acetyl K40) 6-11B-1
Synonyms
,
Product review
Please read our product review about Tubulin (acetyl K40): Antibody Panel to Tubulin Isoforms.
Product Description
Mouse anti Tubulin (acetyl K40) 6-11B-1, Presentation: Ascites. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunodiffusion ( ID ), Western blot / Immunoblot ( WB ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunodiffusion ( ID ), Western blot / Immunoblot ( WB ), Western blot / Immunoblot ( WB ) |
| Reactivity | Mamalia ( Mam ), Chicken ( Chk ), Xenopus ( Xen ), Plant ( Plant ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgG2b |
| Clone | 6-11B-1 |
| Catalog Number | NB600-567 |
| Quantity | 0.1 ml |
| Price | US$ 445 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/p ... |
Datasheet Extract
| Mouse Monoclonal anti-Tubulin (6-11B-1) [Lys40] | |
| Alternate names | anti-Alpha tubulin ubiquitous antibody, anti-H2 ALPHA antibody, anti-TUBA1 antibody, anti-Tubulin alpha 1 chain antibody, anti-Tubulin alpha antibody, anti-Tubulin alpha ubiquitous chain antibody, anti-Tubulin K alpha 1 antibody |
| Background | The major building block of microtubules is tubulin, an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta, that assemble into microtubules. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta tubulins consist of various isotypes. In addition, both undergo post-translational modifications, including acetylation, phosphorylation detyrosination, polyglutamylation, and polyglycylation. Polyglutamylation of tubulin consists of the addition of a lateral chain of glutamyl units on the C-terminal region of tubulin polypeptides. This modification was shown to regulate the interaction between tau, one of the major neuronal microtubule associated proteins, and tubulin. Furthermore, it was shown that injection of monoclonal antibodies specific to the polyglutamylated form of tubulin into HeLa cells caused the disappearance of centrioles, an organelle known to have the most stable microtubules. The detection, localization, and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport, and cytoskeletal functions. Antibodies reacting specifically with the modified forms of alpha and beta tubulin isotypes serve as essential tools in the detection and study of the functional significance of these molecules.
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| Immunogen | Acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin) |
| Format | Purification: Ascites Purity: Ascites BufferSystem: Constituents: Ascites fluid |
| Applications | ELISA , immunohistochemistry , Western Blot 1/2000, RIA, Dot |
| Specificity | Species: Cross-reacts with Protista, Plant, Human, Mouse, Rat, Xenopus laevis, Cow, Chicken, Hamster, Pig and Monkey. Not yet tested in other species. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| References | 1. LeDizet M & Piperno G Detection of acetylated alpha-tubulin by specific antibodies. <i>Methods Enzymol</i> <b>196</b>:264-74 (1991). <PUBMED:2034123>
2. Piperno G <i>et al.</i> Microtubules containing acetylated alpha-tubulin in mammalian cells in culture. <i>J Cell Biol</i> <b>104</b>:289-302 (1987). <PUBMED:2879846> 3. LeDizet M & Piperno G Identification of an acetylation site of Chlamydomonas alpha-tubulin. <i>Proc Natl Acad Sci U S A</i> <b>84</b>:5720-4 (1987). <PUBMED:2441392> 4. Piperno G & Fuller MT Monoclonal antibodies specific for an acetylated form of alpha-tubulin recognize the antigen in cilia and flagella from a variety of organisms. <i>J Cell Biol</i> <b>101</b>:2085-94 (1985). <PUBMED:2415535> |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB600-567 Tubulin (acetyl K40)'.
