NB600-501 Actin (beta) / ACTB antibody
see related secondary antibodies
0.1 ml / US$ 485 no delivery possible
Quick Overview
Mouse anti Actin (beta) / ACTB AC-15
Synonyms
Loading Control, Actin cytoplasmic 1, Beta-actin, , Actin, Cytoplasmic 1
Product review
Please read our product review about Actin (beta) / ACTB : Antibodies to Loading Controls.
Product Description
Mouse anti Actin (beta) / ACTB AC-15, Presentation: Ascites. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Feline ( Fe ), Chicken ( Chk ), Bovine ( Bov ), Canine ( Can ), Drosophila ( Dros ), Fish ( Fish ), Genua Pig ( GP ), Human ( Hu ), Mouse ( Ms ), Por ( Por ), Rabbit ( Rb ), Rat ( Rt ), Sheep ( Sh ), Mamalia ( Mam ) |
| Presentation | Ascites |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | AC-15 |
| Catalog Number | NB600-501 |
| Swiss Prot. No. | P60709 |
| Quantity | 0.1 ml |
| Price | US$ 485 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet | view  NB600-501.pdf |
Datasheet Extract
| Mouse Monoclonal anti-Beta-actin (AC-15) | |
| Alternate names | Anti-A X actin like protein antibody, Anti-Actx antibody, Anti-Beta cytoskeletal actin antibody, Anti-Melanoma X actin antibody, Anti-PS1TP5BP1 antibody, Anti-Beta-actin antibody, Anti-Beta actin antibody, Anti-Actin, beta antibody |
| Concentration | 2.8 mg/ml |
| Background | This is an ideal reagent for use as a cell extract loading control on Western blot. It has been used as such in most of the references below. Molecular weight of beta actin is 42kD. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta). Swiss Num.: P60709 |
| Format | Purification: Ascites Purity: Ascites |
| Applications | ELISA , immunohistochemistry , immunofluorescence , Western Blot 1:5000, Immunocytochemistry 1:1000, Acetone Fixation |
| Specificity | Species: Cross-reacts with Cat, Chicken, Cow, Dog, Drosophila melanogaster, Fish (carp and goldfish), Guinea Pig, Human, Leech, Mouse, Pig, Rabbit, Rat, and Sheep. Detects beta actin in a wide variety of tissues and species. Does not cross react with adult cardiac or skeletal muscle or amoeba beta actin. Not yet tested in other species. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| References | 1. North, A.J., et al. J. Cell Sci. 107, 445-455, (1994)
2. Gimona, M., et al. Cell Motil., 108-116, (1994) 3. Gimona, M., et al., â-actin specific monoclonal antibody. Cell. Motil. Cytoskel. 27, 108-116, (1994) 4. North, A.J., et al., Actin isoform compartments in chicken gizzard smooth muscle cells. J. Cell Sci. 107, 445-455, (1994) 5. North, A.J., et al., Calponin is localized in both the contractile apparatus and the cytoskeleton of smooth muscle cells. J. Cell Sci. 107, 437-444, (1994) 6. Liao, J., et al., Direct reprobing with anti-â-actin antibody as an internal control for western blotting analysis. Biotechniques 28, 216, (2000) 7. Abramovich, D., Parborell, F., and Tesone, M. (2006) Effect of a Vascular Endothelial Growth Factor (VEGF) Inhibitory Treatment on the Folliculogenesis and Ovarian Apoptosis in Gonadotropin-Treated Prepubertal Rats, 75, 434-441. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
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