NB600-313 GFP antibody
see related secondary antibodiessee all 73 GFP products
0.1 mg / US$ 445 no delivery possible
Quick Overview
Rabbit anti GFP -
Synonyms
Green Fluorescent Protein, ,
Product review
Please read our product review about GFP: Antibody Panel to Green Fluorescent Protein (GFP).
Product Description
Rabbit anti GFP -, Presentation: HRP. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Western blot / Immunoblot ( WB ) |
| Presentation | HRP |
| Host | Rabbit |
| Catalog Number | NB600-313 |
| Swiss Prot. No. | P42212 |
| Quantity | 0.1 mg |
| Price | US$ 445 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet | view  NB600-313.pdf |
Datasheet Extract
| Goat Polyclonal anti-GFP, conjugated HRP | |
| Alternate names | anti-Green Fluorescent Protein antibody |
| Concentration | 1 mg/ml |
| Background | Green fluorescence protein (GFP) is a protein derived from the jellyfish Aquorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein-protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology.
YFP differs from GFP due to a mutation at T203Y. Antibodies raised against full-length GFP should also detect YFP and other variants. |
| Immunogen | The immunogen is a Green Fluorescent Protein usion protein corresponding to the full length amino acid sequence (246 residue) derived from the jellyfish Aequorea victoria.
Swiss Num.: P42212 |
| Format | Purification: Immunogen affinity purified Purity: Immunogen affinity purified BufferSystem: 0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2 with 1% BSA, 50% glycerol |
| Applications | ELISA 1:10,000-1:50,000, immunohistochemistry 1:500-1:3,000, Western Blot 1:1,000-1:5,000 |
| Specificity | Species: No reaction with human, mouse, or rat. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| References | 1. Alvarez, B. et al. Molecular cloning, characterization and tissue expression of porcine Toll-like receptor 4. Dev Comp Immunol 30:345-55 (2006). PubMed: 16139355
2. Conticello, SG. et al. The Vif protein of HIV triggers degradation of the human antiretroviral DNA deaminase APOBEC3G. Curr Biol 13:2009-13 (2003). PubMed: 14614829 |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB600-313 GFP'.