NB600-303 GFP antibody
see related secondary antibodiessee all 73 GFP products
50 µl / US$ 555 no delivery possible
Quick Overview
Rabbit anti GFP -
Synonyms
Green Fluorescent Protein, ,
Product review
Please read our product review about GFP: Antibody Panel to Green Fluorescent Protein (GFP).
Product Description
Rabbit anti GFP -, Presentation: Serum. Product is tested for ChIP assay ( ChIP ), Immunofluorescence ( IF ), Frozen sections ( C ), Paraffin Sections ( P ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | ChIP assay ( ChIP ), Immunofluorescence ( IF ), Frozen sections ( C ), Paraffin Sections ( P ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | NB600-303 |
| Swiss Prot. No. | P42212 |
| Quantity | 50 µl |
| Price | US$ 555 no delivery possible |
| Delivery | Europe only |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet | view  NB600-303.pdf |
Datasheet Extract
| Rabbit Polyclonal anti-GFP | |
| Alternate names | anti-Green Fluorescent Protein antibody, anti-EGFP antibody, anti-YFP antibody |
| Concentration | 6.2 mg/ml |
| Background | A highly versatile antibody that gives a stronger signal than other anti-GFP antibodies available. On Western blot the antibody detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins and has also been shown to be useful on mouse sections fixed with formalin. In immunocytochemistry, the antibody gives a very good signal on recombinant YES-GFP chimeras expressed in COS cells. It is routinely used in immunoprecipitation (IP) and IP-Western protocols and has been used successfully in HRP-immunohistochemistry (1:200) on whole-mount mouse embryos. |
| Immunogen | Highly purified recombinant full length protein made in E. coli. The antibody is directed against the entire GFP molecule. Swiss Num.: P42212 |
| Format | Purification: Whole antisera Purity: Whole antisera BufferSystem: 0.02M KPO4, 0.15M NaCl [pH 7.2] |
| Applications | immunoprecipitation **, Western Blot 1:2,500 - 1:10,000, Immunocytochemistry (Use in 4% normal serum) 1:200 - 1/1000, Immunohistochemistry-Paraffin 1:200 *, Immunogold Electron Microscopy 1:100 |
| Specificity | Species: Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| References | 1. Zhang, Z., Shao, S., and Meistrich, M. L. Irradiated Mouse Testes Efficiently Support Spermatogenesis Derived From Donor Germ Cells of Mice and Rats. Biol. of Reprod. 27: 365-375, 2006.
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12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB600-303 GFP'.