NB300-323 Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
see related secondary antibodiessee all 49 Glyceraldehyde-3-Phosphate Dehydrogenase products
0.1 ml / US$ 485 no delivery possible
Quick Overview
Rabbit anti Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Synonyms
Loading Control, GAPD, G3P, ,
Product review
Please read our product review about Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH): Antibodies to Loading Controls.
Product Description
Rabbit anti Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) . Product is tested for Western blot / Immunoblot ( WB )
Properties
| Applications | Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Yeast ( Yeast ) |
| Host | Rabbit |
| Catalog Number | NB300-323 |
| Swiss Prot. No. | P04406 |
| Quantity | 0.1 ml |
| Price | US$ 485 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/p ... |
Datasheet Extract
| Rabbit Polyclonal anti-GAPDH | |
| Alternate names | anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody, anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685 antibody |
| Concentration | 0.2 mg/ml |
| Background | GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important house keeping functions, levels of GAPDH mRNA are often used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH as loading controls for western blotting experiments. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations with actin and tubulin have also be reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic. |
| Immunogen | The epitope recognized by this antibody maps to a region between residues 200 and 250 of human Glyceraldehyde-3-Phosphate Dehydrogenase using the numbering given in entry NP_002037.2 (GeneID 2597). Swiss Num.: P04406 |
| Format | Purification: Immunogen affinity purified Purity: Immunogen affinity purified BufferSystem: Tris-buffered Saline containing 0.1% BSA |
| Applications | Western Blot |
| Specificity | Species: Human and Mouse. Reactivity with X. laevis and S. Pombe and S. cerevisae GAPDH is expected owing to conservation of sequence between this ortholog and Human GAPDH at the site to which the epitope maps. |
| Storage | Store at 4C. Do not freeze. |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||