NB110-85544 OCT4 antibody
see related secondary antibodiessee all 12 OCT4 products
0.1 mg / US$ 525 no delivery possible
Quick Overview
Rabbit anti OCT4
Synonyms
POU5F1, OCT3, OCT4, OTF3, OCT-3, OCT-4, OTF-3,
Product Description
Rabbit anti OCT4 , Presentation: Aff - Purified. Product is tested for Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Bovine ( Bov ), Canine ( Can ), Equine ( Eq ), Human ( Hu ), Monkey ( Mky ), Mouse ( Ms ), Por ( Por ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | NB110-85544 |
| Quantity | 0.1 mg |
| Price | US$ 525 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/d ... |
Datasheet Extract
| Rabbit Polyclonal anti-Oct4 | |
| Alternate names | anti-MGC22487 antibody, anti-Oct 3 antibody, anti-Oct 4 antibody, anti-OCT3 antibody, anti-Oct4 antibody, anti-Octamer binding transcription factor 3 antibody, anti-OTF3 antibody, anti-OTF4 antibody, anti-POU domain class 5 transcription factor 1 antibody, anti-POU5F1 antibody |
| Concentration | 1.0 mg/ml |
| Background | Oct-4, also known as POU domain transcription factor, Octomerbinding transcription factor 3 and Oct-3, is expressed in human and mouse totipotent embryonic stem and germ cells. It may function in preventing these cells from differentiating. Each type of established mammalian pluripotent stem cell line (embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells) expresses Oct-4, which disappears rapidly when the cells differentiate. Most studies on Oct-4 have been performed on murine embryos. In human oocytes of various maturity and normally developing preimplantation/cleavage embryos, a variable Oct-4 expression pattern has been revealed, concomitant with a pure cytoplasmic localization of the protein. This variability in expression fades away during compaction, indicating embryonic Oct-4 expression; the protein appears in the nucleus, implying biological activity. Functional Oct-4 has been found in human amniotic fluid. As Oct-4 transcripts have also been found in various differentiated cells, the presence of Oct-4 transcripts or proteins may not be sufficient for identifying undifferentiated cell lines in humans. It may also be important to examine the localization of Oct-4 proteins within a cell. Oct-4 has been shown to interact with Nanog and with proteins from multiple repression complexes, including the NuRD, Sin3A and Pml complexes, possibly to control ES fate. Reactivation of Oct-4 expression is postulated to occur in differentiated cells that have undergone carcinogenesis, or tumor formation. |
| Immunogen | This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein. |
| Format | Purification: Affinity purified Purity: Affinity purified BufferSystem: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Applications | ELISA 1:350000, immunofluorescence User Optimized, immunoprecipitation User Optimized, Western Blot 1:1000-1:1500, |
| Specificity | Species: This antibody is reactive to Human, Mouse, Chimpanzee, Macaque and Horse. Expected to react to Mink, Dog, Bovine and Pig baised on sequence homology. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Prusa,A-R, Marton,E, Rosner,M, et al. Oct-4-expressing cells in human amniotic fluid: a new source for stem cell research? Human Reproduction 2003; 18 (7), 1489-1493 2. Cauffman,G., Van de Velde,H., Liebaers,I., and Van Steirteghem,A. Oct-4 mRNA and protein expression during human preimplantation development Molecular Human Reproduction 2005; 11(3),173-181 3. Donovan, P.J. High Oct-ane fuel powers the stem cell. Nature Genet. 2001; 29, 246û247 4. Pesce, M. and Schöler, H.R. Oct-4: gatekeeper in the beginnings of mammalian development. Stem Cells 2001; 19 (4), 271û278. 5. Lin,L., Czerwiec,E., Keefe,D.L. Effect of ploidy and parental genome composition on expression of Oct-4 protein in mouse embryos. Gene Expression Patterns 2004; 4 (4), 433-441 |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB110-85544 OCT4'.
