NB110-55438 ABCG1 antibody
see related secondary antibodiessee all 10 ABCG1 products
0.1 ml / US$ 515 no delivery possible
Quick Overview
Rabbit anti ABCG1 EP1366Y
Synonyms
, White protein homolog, ABC8, WHT1, ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8
Product Description
Rabbit anti ABCG1 EP1366Y, Presentation: Aff - Purified. Product is tested for Flow cytometry ( F ), Immunofluorescence ( IF ), Western blot / Immunoblot ( WB )
Properties
| Applications | Flow cytometry ( F ), Immunofluorescence ( IF ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Isotype | IgG |
| Clone | EP1366Y |
| Catalog Number | NB110-55438 |
| Swiss Prot. No. | P45844 |
| Quantity | 0.1 ml |
| Price | US$ 515 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/d ... |
Datasheet Extract
| Rabbit Monoclonal anti-ABCG1 (EP1366Y) | |
| Alternate names | anti-Abc8 antibody, anti-ATP-binding cassette sub-family G member 1 antibody, anti-ATP-binding cassette transporter 8 antibody, anti-White protein homolog antibody, anti-wht1 antibody |
| Background | ATP-binding cassette (ABC) transporter ABCG1 (ABC8) is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Conversely, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1. Immunocytochemical and flow cytometric analyses revealed that ABCG1 is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages. Results demonstrate a regulator function for ABCG1 in cholesterol and phospholipid transport define a biologic activity for ABC transporters in macrophages (1). ABC transporters belonging to the ABCG (White) subfamily (ABCG1, ABCG5, and ABCG8) have been shown to be critically involved in the regulation of lipid-trafficking mechanisms in macrophages, hepatocytes, and intestinal mucosa cells (2) Studies show that treatment with Rhodobacter sphaeroiders LPS, an LPS antagonist, or PD169316, a specific p38 MAP kinase inhibitor, prevented the induction of ABCA1 by LPS. Therefore, this suggests that both transport of LPS from the plasma membrane to an intracellular site and activation of p38 MAP kinase are involved in the LPS-mediated induction of ABCA1 (3). |
| Immunogen | A synthetic peptide corresponding to residues on human ABCG1. Swiss Num.: P45844 |
| Format | Purification: Tissue culture supernatant Purity: Tissue culture supernatant BufferSystem: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Applications | immunohistochemistry 1:50 ? 100, Western Blot 1:2000, Immunocytochemistry 1:100 - 250 |
| Specificity | Species: Human, Mouse, Rat. Not tested in other species. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Klucken J, et al. Proc Natl Acad Sci 97(2):817-22, 2000
2. Kaplan R, et al. J Lipid Res 43(6):952-9, 2002. 3. Schmitz G, et al. J Lipid Res 42(10):1513-20, 2001 |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB110-55438 ABCG1'.
