NB100-690 Tubulin alpha antibody
see related secondary antibodiessee all 56 Tubulin alpha products
0.1 mg / US$ 485 no delivery possible
Quick Overview
Mouse anti Tubulin alpha DM1A
Synonyms
Microtubule Marker, Loading Control, alpha-Tubulin, , ,
Product review
Please read our product review about Tubulin alpha: Antibodies to Loading Controls.
Product Description
Mouse anti Tubulin alpha DM1A, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Chicken ( Chk ), Bovine ( Bov ), Genua Pig ( GP ), Human ( Hu ), Mouse ( Ms ), Por ( Por ), Rat ( Rt ), Xenopus ( Xen ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | DM1A |
| Catalog Number | NB100-690 |
| Quantity | 0.1 mg |
| Price | US$ 485 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/p ... |
Datasheet Extract
| Mouse Monoclonal anti-alpha Tubulin (DM1A) | |
| Alternate names | anti-FLJ30169 antibody, anti-H2 ALPHA antibody, anti-Testis specific alpha tubulin antibody, anti-TUBA1 antibody, anti-Tubulin alpha 1 chain antibody, anti-
Tubulin H2 alpha antibody |
| Concentration | 0.2 mg/ml |
| Background | Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Alpha tubulin is not suitable as loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1):53-60) in cells undergoing adipose differentiation actin synthesis decreases by 90%. Excellent as a protein loading control antibody. |
| Immunogen | Native chicken brain microtubules. |
| Format | Purification: protein G purified Purity: protein G purified BufferSystem: PBS |
| Applications | immunofluorescence , immunoprecipitation , Western Blot , Immunohistochemistry-Paraffin |
| Specificity | Species: Cross-reacts with Chicken, Cow, Gerbil, Guinea pig, Human, Mouse, Pig, Rat, and Xenopus laevis. Not yet tested in other species. |
| Storage | Store at 4C. Do not freeze. |
| References | Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintainance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
Alpha tubulin is not suitable as loading control in adipose tissue as expression of tubulin in adipose tissue is very low ( Spiegelman and Farmer, Cell, 1982, 29(1):53-60) in cells undergoing adipose differentiation actin synthesis decreases by 90%. Excellent as a protein loading control antibody. 1. Odreman F et al. Proteomic studies on low- and high-grade human brain astrocytomas. J Proteome Res 4:698-708 (2005). PubMed: 15952716 2. Roychowdhury S et al. G protein binding and G protein activation by nucleotide transfer involve distinct domains on tubulin: regulation of signal transduction by cytoskeletal elements. Biochemistry 32:4955-61 (1993). PubMed: 8490031 3. Berry S et al. The role of microtubules in platelet secretory release. Biochim Biophys Acta 1012:46-56 (1989). PubMed: 2730897 4. Breitling F & Little M Carboxy-terminal regions on the surface of tubulin and microtubules. Epitope locations of YOL1/34, DM1A and DM1B. J Mol Biol 189:367-70 (1986). PubMed: 2427729 5. Blose SH et al. 10-nm filaments are induced to collapse in living cells microinjected with monoclonal and polyclonal antibodies against tubulin. J Cell Biol 98:847-58 (1984). PubMed: 6538204 |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-690 Tubulin alpha'.
