NB100-616 p300 antibody
see related secondary antibodiessee all 28 p300 products
0.2 mg / US$ 435 no delivery possible
Quick Overview
Mouse anti p300 RW105
Product Description
Mouse anti p300 RW105, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Mouse ( Ms ), Rat ( Rt ), Monkey ( Mky ), Mink ( Mink ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | RW105 |
| Catalog Number | NB100-616 |
| Quantity | 0.2 mg |
| Price | US$ 435 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet | view  NB100-616.pdf |
Datasheet Extract
| Mouse Monoclonal anti-p300 (RW105) | |
| Alternate names | anti-CREBBP/EP300 inhibitory protein 1 antibody; anti-Cyclic AMP responsive enhancer binding protein antibody; anti-EP300: E1A binding protein p300 antibody; anti-RB and P300 binding protein EID 1 antibody; anti-Retinoblastoma protein associated protein antibody |
| Concentration | 1 mg/ml |
| Background | p300 is a relatively stable, ubiquitously expressed, nuclear phosphoprotein which is conserved among a variety of mammalian species. It is actively synthesized and phosphorylated in both quiescent and proliferating cells.
Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators and have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes. As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription. |
| Immunogen | Fusion protein containing residues 1572-2371 of human p300. |
| Format | BufferSystem: PBS [pH7.4] |
| Applications | immunofluorescence , immunoprecipitation , Western Blot 1:250-1:500 |
| Specificity | Species: This antibody reacts with human, mouse, rat, monkey, and minkprotein. Other species have not been tested. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | 1. Eckner, R., et al. Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor. Genes & Dev., 8: 869-884 (1994)
2. Eckner, R., et al. Association of p300 and CBP with simian virus 40 large T antigen. Mol. & Cell Bio., 16: 3454-3464 (1996) 3. Eckner, R., et al. Interaction and functional collaboration of p300-CBP anf bHLH proteins in muscle and B-cell differentiation. Genes & Dev., 10: 2478-2490 (1996) |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-616 p300'.
