NB100-2141 Catenin beta antibody
see related secondary antibodiessee all 65 Catenin beta products
0.1 ml / no delivery possible
Quick Overview
Rabbit anti Catenin beta
Synonyms
Beta-catenin, Catenin beta-1, CTNNB1, CTNNB, ,
Product review
Please read our product review about Catenin beta: Antibodies to Catenins.
Product Description
Rabbit anti Catenin beta , Presentation: Serum. Product is tested for Frozen sections ( C ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Western blot / Immunoblot ( WB ) |
| Reactivity | Chicken ( Chk ), Human ( Hu ), Mouse ( Ms ), Por ( Por ), Rat ( Rt ), Xenopus ( Xen ), Zebrafish ( Zebrafish ) |
| Presentation | Serum |
| Host | Rabbit |
| Catalog Number | NB100-2141 |
| Swiss Prot. No. | P35222 |
| Quantity | 0.1 ml |
| Price | no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/p ... |
Datasheet Extract
| Rabbit Polyclonal anti-Catenin beta | |
| Alternate names | Anti-Beta catenin antibody, Anti-Cadherin associated protein antibody, Anti-Catenin beta 1 antibody, Anti-CATNB antibody, Anti-CTNNB antibody, Anti-CTNNB1 antibody |
| Background | The distinct peripheral cytosolic proteins, a-, b- and g-catenin (102 kDa, 94 kDa and 86 kDa respectively), are found in varying abundance in many developing and adult tissues.1,2,3 The catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the celladhesion cadherins. Cadherins are transmembrane cell surface glycoprotein molecules, concentrated at adherens junctions that mediate calcium-dependent
intercellular interactions and are important for tissue morphogenesis.4 The linkage of the epithelial Ecadherin/ uvomorulin to actin is essential for the cell binding function of this cadherin. Catenins also link E cadherin to other integral membrane proteins such as Na+/K+-ATPase, or to cytoplasmic proteins such as fodrin, ankyrin, Src and Yes kinases5 and are modulated by Wnt-1 protooncogene.6,7 They are considered good candidates for mediating transduction of cell-cell contact positional signals to the cell interior.4,5 Within its conserved regions a-catenin shows 30% identity to vinculin, a protein found mainly in focal cell-cell and cell substrate adhesions.2,3 Vinculin is known to interact with a-actinin, which in turn is associated with actin filaments in their site of attachment to the cell membrane focal contacts. The protein, a-catenin, is capable of interacting with N-cadherin and P-cadherin. Absence of a-catenin is found in certain tumor cell lines.8 Frequent reduction of a-catenin levels in human carcinomas of the esophagus, stomach and colon is reported9 Enhancement of tumor cell invasion and metastatic ability of such cells following catenins down-regulation is speculated. Prostate cancer development appears to be correlated with a-catenin gene deletions. Plakoglobin (probably identical to g-catenin) and b-catenin are structural and possibly functional mammalian homologues of armadillo (arm), a Drosophila protein involved in signal transduction. The protein, b-catenin, binds directly to the cytoplasmic tail of Ecadherin. It binds to the amino terminus of a-catenin and also interacts with the cytosolic protein product of the human tumor suppressor gene APC.10 Mutations in this gene occur early in colon carcinogenesis. Such mutations are linked to familial adenomatous polyposis and to progression of sporadic colorectal and gastric tumors. The preferential interaction of b-catenin with the APC protein involves a 15-amino acid repeat in the latter11 and b-catenin cell levels seem to be controlled by APC.12 The central core region of b-catenin is involved in mediation of the interaction of cadherin-catenin complex with the epidermal growth factor receptor.13 The protein, b-catenin, is the target of two signal transduction pathways mediated by the protooncogenes Src and Wnt-1. p120cas which exhibits structural similarity to b-catenin and plakoglobin may represent another catenin associated with cadherin.14 Polyclonal antibodies against defined type-specific catenin peptides are useful tools for the study of these proteins. Such antibodies recognize the respective catenin type in a variety of immunological techniques such as immunoprecipitation, immunoblotting, immunofluorescence and immunoperoxidase in different cell types from various species. |
| Immunogen | Synthetic peptide: PGDSNQLAWFDTDL conjugated to KLH, corresponding to amino acids 768-781 of Human or mouse, Catenin. Swiss Num.: P35222 |
| Format | Purification: Whole antisera Purity: Whole antisera BufferSystem: Whole Serum |
| Applications | Western Blot 1/4000, Immunohistochemistry-Frozen 1/2000, Dot:Assay dependent |
| Specificity | Species: Cross-reacts with Chicken, Human, Mouse, Pig, Rat, Xenopus laevis and Zebrafish. Expected to cross-react with a wide range of species due to sequence homology. Not yet tested in other species. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| References | 1. Nagafuchi, A., and Takeichi, M., Cell Regul., 1, 37(1989).
2. Ozawa, M., et al., EMBO J., 8, 1711 (1989). 3. Ozawa, M., et al., Proc. Natl. Acad. Sci. USA, 87,4246 (1990). 4. Cowin, P., Proc. Natl. Acad. Sci. USA, 91, 11989(1994). 5. Tsukita S., et al., J. Cell Biol., 123, 1049 (1993). 6. Bradley, R., et al., J. Cell Biol., 123, 1857 (1994). 7. Hinck, L., et al., J. Cell Biol., 124, 729 (1994). 8. Cowin, P., et al., Cell, 46, 1063 (1986). 9. Shiozaki, H., et al., Am. J. Pathol., 144, 667 (1994). 10. Sch |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-2141 Catenin beta'.