NB100-1629 E2F1 Transcription Factor antibody
see related secondary antibodiessee all 43 E2F1 Transcription Factor products
0.1 ml / US$ 515 no delivery possible
Quick Overview
Rabbit anti E2F1 Transcription Factor
Synonyms
E2F-1, RBBP3, RBBP-3, PBR3, RBAP-1, RBAP1, , Retinoblastoma-binding protein 3, PRB-binding protein E2F-1, Retinoblastoma-associated protein 1
Product review
Please read our product review about E2F1 Transcription Factor: Antibodies to E2F Transcription Factor Family.
Product Description
Rabbit anti E2F1 Transcription Factor , Presentation: Aff - Purified. Product is tested for Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Aff - Purified |
| Host | Rabbit |
| Catalog Number | NB100-1629 |
| Swiss Prot. No. | Q01094 |
| Quantity | 0.1 ml |
| Price | US$ 515 no delivery possible |
| Delivery | Not USA/Canada |
| Manufacturer | Novus Biologicals Inc. |
| Datasheet |  http://www.novusbio.com/p ... |
Datasheet Extract
| Rabbit Polyclonal anti-E2F1 | |
| Alternate names | anti-E2F 1 antibody, anti-E2F transcription factor 1 antibody, anti-PBR 3 antibody, anti-PBR3 antibody, anti-PRB binding protein E2F 1 antibody, anti-PRB-binding protein E2F-1 antibody, anti-RBAP 1 antibody, anti-RBBP 3 antibody, anti-RBBP3 antibody, anti-RBP 3 antibody, anti-RBP3 antibody, anti-Retinoblastoma associated protein 1 antibody, anti-Retinoblastoma binding protein 3 antibody, anti-Transcription factor E2F1 antibody |
| Background | Anti-E2F1 reacts specifically with the E2F1 transcription regulator protein (recombinant). The antibody may be used for immunoblotting (~50 kDa) and immunoprecipitation techniques using whole cell extracts of E2F1 transfected cells, or in vitro translated E2F1 proteins. Staining of the E2F1 band by immunoblotting is specifically inhibited with the immunizing E2F1 peptide. The E2F family of transcription factors is important for cell cycle control. The E2F transcription factors are heterodimeric molecules composed of two distinct proteins termed E2F and DP. The E2F component of this protein complex is encoded by a family of genes consisting of at least 6 members (E2F-1 to -6). The DP family contains three proteins (DP -1 to -3), which heterodimerize interchangeably with any of the E2F
proteins. E2F/DP target genes include genes important for DNA replication (e.g. DHFR, ORC1, MCM), cyclin genes (A and E), proto-oncogene (myc, myb) and the Rb family genes (Rb, p107). The E2F/DP transcription factors are tightly regulated by multiple regulatory mechanisms. Besides being regulated by the combination of the two components, the E2F/DP complex is negatively regulated by binding to one or more members of the retinoblastoma (Rb) pocket protein family: pRb, p107 and p130. Complexes of unphosphorylated pRb and E2F/DP act as transcriptional repressors that contribute to the Rbdependent G1 arrest. Once a pocket protein is phosphorylated by CDKs, the E2F/DP/pocket protein complex dissociates, resulting in free, transcriptionally active E2F/DP heterodimer. Another level of regulation is the synthesis and degradation of the proteins. Certain E2F species are actively degraded by the ubiquitin-proteasome pathway and their synthesis is cell-cycle regulated. The E2F proteins can be grouped into 3 subfamilies based upon structural homology and functional characteristics. The first subfamily consists of E2F-1, -2, and ?3 that bind to and are regulated only by pRb. All members of this group of proteins contain a dedicated cyclin binding sequence N-terminal to the DNA binding domain that mediates the binding of cyclin A/cdk2. The second family consists of E2F-4 and -5, which bind mainly to p107 and p130 and sometimes to pRb in the case of E2F-41. E2F-6 (also known as EMA) seems to belong to another subfamily because it does not bind any of the pRb family members (it lacks a pocket protein-binding domain) and its overexpression seems to delay exit from S-phase rather than induce Sphase. 4 E2F1 synthesis is cell cycle regulated in cells emerging from G0. In growth arrested cells, E2F1 is undetectable but its levels rise during late G1. E2F1 is accumulated in the nuclei. The E2F1 promoter contains E2F DNA binding sites and is cell cycle regulated. Antibodies to E2F1 are a useful tool for the detection and localization of E2F1 protein in cells. |
| Immunogen | Synthetic peptide: KTPSQEVTSEEENRATDSAT conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 310-329 of Human E2F 1. Swiss Num.: Q01094 |
| Format | Purification: Immunogen affinity purified Purity: Immunogen affinity purified BufferSystem: 10mM PBS. [pH 7.4] with 1% BSA |
| Applications | immunoprecipitation, Western Blot |
| Specificity | Species: Cross-reacts with Human. Not yet tested in other species. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| References | Cartwright P et al. E2F-6: a novel member of the E2F family is an inhibitor of E2F-dependent transcription. Oncogene 17:611-23 (1998). |
12 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1364B | Rabbit IgG (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 295 | |||||
| R1364F | Rabbit IgG (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 285 | |||||
| R1364T | Rabbit IgG (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 285 | |||||
| R1364TR | Rabbit IgG (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 295 | |||||
| R1364HRP | Rabbit IgG (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 295 | |||||
| R1364AP | Rabbit IgG (H&L) | ||||||
| Goat | AP | 1 mg / US$ 325 | |||||
| R1458C2 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1458C3 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1458C35 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1458C5 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1458C55 | Rabbit IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1427R | Rabbit IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'NB100-1629 E2F1 Transcription Factor'.
