GTX27179 NIH 3T3 lysate
see all 2 NIH 3T3 lysate products
100 µg / no delivery possible
Quick Overview
NIH 3T3 lysate
Product Description
NIH 3T3 lysate
Properties
| Catalog Number | GTX27179 |
| Quantity | 100 µg |
| Price | no delivery possible |
| Delivery | Europe only |
| Manufacturer | GeneTex Inc. |
| Datasheet |  http://www.genetex.com/co ... |
Datasheet Extract
| NIH 3T3 lysate | |
| Concentration | 2 mg/ml |
| Background | Mouse NIH/3T3 cell lysate was prepared by homogenization in modified RIPA buffer(50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate,1 mM phenylmethyl-sulfonyl flouride, 5 ug/ml of aprotinin, 5 ug/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol. |
| Format | Purification: Whole Cell Lysate Purity: Whole Cell Lysate BufferSystem: Whole cell lysate in 1 X SDS-PAGE sample loading buffer with 5% b-mercaptoethanol |
| Applications | WB |
| Storage | Store at -20?C for up to three months or at |
