GTX14635 A431 Lysate EGF treated
100 µg / no delivery possible
Quick Overview
A431 Lysate EGF treated
Product Description
A431 Lysate EGF treated
Properties
| Catalog Number | GTX14635 |
| Quantity | 100 µg |
| Price | no delivery possible |
| Delivery | Europe only |
| Manufacturer | GeneTex Inc. |
| Datasheet |  http://www.genetex.com/co ... |
Datasheet Extract
| A431 Lysate EGF treated | |
| Concentration | 1 mg/ml |
| Background | Cellular protein preparation from A431 cells containing phosphorylated proteins, including the EGF receptor. A431 cells were cultured for 20 minutes in the presence of 50ng/ml EGF. Cells were lysed in modified RIPA buffer (50mM Tris-HCl, pH7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM sodium chloride, 1mM EDTA, 1mM PMSF, 1ug/ml aprotinin, 1ug/ml leupeptin, 1ug/ml pepstain, 1mM sodium fluoride) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% brophenol blue). |
| Format | State: Liquid Purification: Nuclear Extract Purity: Nuclear Extract |
| Storage | Keep as concentrated solution. Aliquot and store at -20 |
