DM379 p15INK4B antibody
see related secondary antibodiessee all 13 p15INK4B products
1 ml / US$ 605
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti p15INK4B 15P06
Synonyms
CDKN2B, MTS2,
Product Description
Mouse anti p15INK4B 15P06, Presentation: Purified. Product is tested for Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Canine ( Can ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 15P06 |
| Catalog Number | DM379 |
| Quantity | 1 ml |
| Price | US$ 605 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DM379.pdf |
Datasheet Extract
| Background | p15INK4b is a specific inhibitor of cdk4/cdk6 and is periodically expressed during cell cycle. Reportedly, p15INK4b expression is induced approximately 30-fold in human keratinocytes by treatment with TGF-beta, suggesting that p15INK4b may act as an effector of TGF-Beta-mediated cell cycle arrest. p15INK4b is mapped to chromosome 9p21, a region of frequent loss in a wide variety of cancers. |
| Immunogen | BALB/C mice were immunized with purified human recombinant full length p15INK4b |
| Format | State: Liquid Purification: Protein G purified ascites BufferSystem: PBS containing 0.05 % sodium azide as preservative. |
| Applications | Immunoprecipitation. Immunofluorescence. Immunohistochemistry on paraffin embedded sections: 1:25-1:50 in an ABC method, 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Western Blot. Positive Control: Colon carcinoma. |
| Specificity | This antibody reacts with a protein of 15 kD, p15INK4b. Cellular Localization: Nuclear. Species: Human and Dog. |
| Storage | Store the antibody at 2-8°C or long term in aliquots at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Gray-Bablin et al. Cancer Res 57: 604, 1997.
2. Jen et al. Cancer Res 54: 6353, 1994. 3. Naumann et al. Gastroenterol 110: 1215, 1996. 4. Saxena et al. Oncogene 86: 755, 1996. |
| Pictures | Formalin-fixed, paraffin-embedded human colon carcinoma stained with p15 Ab (Cat. DM379) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
|
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'DM379 p15INK4B'.
