DM124 Estrogen Receptor alpha / ESR1 antibody
see related secondary antibodiessee all 119 Estrogen Receptor alpha / ESR1 products
0.5 ml / US$ 565
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Estrogen Receptor alpha / ESR1 1D5
Synonyms
ER-alpha, Estradiol receptor, ESR, NR3A1, , Nuclear receptor subfamily 3 group A member 1, Estrogen receptor, ER alpha, Estrogen receptor 1, Major ORF
Product review
Please read our product review about Estrogen Receptor alpha / ESR1 : Antibodies to Estrogen Receptors and Estrogen-Related Receptors.
Product Description
Mouse anti Estrogen Receptor alpha / ESR1 1D5, Presentation: Supernatant. Product is tested for Frozen sections ( C ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Paraffin Sections ( P ), Western blot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ) |
| Presentation | Supernatant |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | 1D5 |
| Catalog Number | DM124 |
| Swiss Prot. No. | P03372 |
| Quantity | 0.5 ml |
| Price | US$ 565 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  DM124.pdf |
Datasheet Extract
| Background | The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm. However, on cryostat sections a positive staining of estrogen receptor in the nucleus as well as cytoplasm can be seen. |
| Immunogen | Recombinant human estrogen receptor protein. Swiss Num.: P03372 |
| Format | State: Liquid Ig fraction BufferSystem: Contains 0.05% sodium azide as preservative |
| Applications | Western Blot: 1:50 to 1:100
Immunohistochemistry on frozen and paraffin sections: diluted of 1:25 to 1:50 in an ABC method (30 minutes at room temperature) Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive control: Breast carcinoma |
| Specificity | This antibody is specific to estrogen receptor, which is associated with superior prognosis and a better response to endocrine therapy. Species: Human. |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | i) Bur et al. Cancer 69: 1174, 1992.
ii) Graham et al. Am J surg pathol 15: 475, 1991. iii) Kumar et al. Cell 51: 941, 1987. iv) Pertschuk et al. Cancer 66: 1663, 1990. v) Deamant et al. Applied Immunohistochemistry 1: 188, 1993. |
| Pictures | Formalin fixed paraffin embedded human breast carcinoma stained with Estrogen Receptor antibody DM124 |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'DM124 Estrogen Receptor alpha / ESR1 '.
