BM753S Tubulin alpha antibody
see related secondary antibodiessee all 55 Tubulin alpha products
0.1 mg / US$ 315
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Tubulin alpha TU-01
Synonyms
Microtubule Marker, Loading Control, alpha-Tubulin, , ,
Product review
Please read our product review about Tubulin alpha: Antibodies to Loading Controls.
Product Description
Mouse anti Tubulin alpha TU-01, Presentation: Purified. Product is tested for Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Enzyme Immunoassay ( E ), Immunofluorescence ( IF ), Immunoprecipitation ( IP ), Western blot / Immunoblot ( WB ) |
| Reactivity | All Species ( All Species ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | TU-01 |
| Catalog Number | BM753S |
| Quantity | 0.1 mg |
| Price | US$ 315 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM753S.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening; this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or morecommonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight about 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression.
Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structuraldomain. |
| Immunogen | Fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization |
| Format | State: Liquid IgG fraction Purification: Precipitation methods ( >95% by SDS-PAGE) BufferSystem: PBS with 15 mM sodium azide as preservative, pH 7.4 |
| Applications | Western Blotting (recommended dilution: 1-2 µg/ml, incubation 60 min, room temperature)
Positive control: HPB-ALL peripheral blood leukemia cell lysate (incubation 60 min), Porcine brain lysate (incubation 90 min) Sample preparation: Laemmli reducing buffer Application note: Reducing conditions. Immunocytochemistry (recommended dilution: DY547 conjugate: 2-3 µg/ml, FITC conjugate: 3 µg/ml). Staining technique: fixed and permeabilized cells. Clone TU-01 has also been described to work in immunohistochemistry, ELISA and immunoprecipitation. |
| Specificity | The antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha tubulin.
Reacts with all species (recognized epitope conserved within all species). |
| Storage | Stable at 2-8°C for up to one month or at -20°C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. |
| References | Viklicky V. et al., Cell Biol. Int. Rep. 6, 725 (1982).
Draber P. et al., Eur. J. Cell Biol. 41, 82 (1986). Grimm M. et al., Biochim. Biophys. Acta 914, 83 (1987). Draber P. et al., J. Cell Sci. 92, 519 (1989). Draber et al., Histochemistry 95, 519 (1991). Linhartova I. et al., Biochem. J. 288, 919 (1992). |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM753S Tubulin alpha'.
