BM753F Tubulin alpha antibody
see related secondary antibodiessee all 55 Tubulin alpha products
0.1 mg / US$ 365
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Tubulin alpha TU-01
Synonyms
Microtubule Marker, Loading Control, alpha-Tubulin, , ,
Product review
Please read our product review about Tubulin alpha: Antibodies to Loading Controls.
Product Description
Mouse anti Tubulin alpha TU-01, Presentation: FITC. Product is tested for Immunofluorescence ( IF )
Properties
| Applications | Immunofluorescence ( IF ) |
| Reactivity | All Species ( All Species ) |
| Presentation | FITC |
| Host | Mouse |
| Isotype | IgG1 |
| Clone | TU-01 |
| Catalog Number | BM753F |
| Quantity | 0.1 mg |
| Price | US$ 365 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM753F.pdf |
Datasheet Extract
| Concentration | 1.0 mg/ml |
| Background | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight about 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression.
Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain. |
| Immunogen | Fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization |
| Format | State: Liquid purified Ig fraction BufferSystem: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. |
| Applications | Immunoflourescence (1/50 on fixed and permeabilized cells). |
| Specificity | The antibody recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin in all species (recognized epitope conserved within all species). |
| Storage | Store the antibody undiluted at 4-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch. |
| References | Viklicky V, Draber P, Hasek J, Bartek J.: Production and characterization of a monoclonal antitubulin antibody. Cell Biol Int Rep. 1982 Aug;6(8):725-31.
Draber P, Draberova E, Zicconi D, Sellitto C, Viklicky V, Cappuccinelli P.: Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin. Eur J Cell Biol. 1986 Jun;41(1):82-8. Grimm M, Breitling F, Little M.: Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1. Biochim Biophys Acta. 1987 Jul 24;914(1):83-8. Draber P, Draberova E, Linhartova I, Viklicky V.: Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies. J Cell Sci. 1989 Mar;92 ( Pt 3):519-28. Draber P, Draberova E, Viklicky V.: Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta tubulin epitope in the sperm head. Histochemistry. 1991;95(5):519-24. Linhartova I, Draber P, Draberova E, Viklicky V.: Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 ( Pt 3):919-24. Novakova M, Draberova E, Schürmann W, Czihak G, Viklicky V, Draber P.: gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil Cytoskeleton. 1996;33(1):38-51. Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P.: Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells. Planta. 1997;201 (3):349-58. Kukharskyy V, Sulimenko V, Macurek L, Sulimenko T, Draberova E, Draber P.: Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells. Exp Cell Res. 2004 Aug 1;298(1):218-28. |
14 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1253B | Mouse IgG (H&L) | ||||||
| Rabbit | Biotin | 2 mg / US$ 305 | |||||
| R1253F | Mouse IgG (H&L) | ||||||
| Rabbit | FITC | 2 mg / US$ 285 | |||||
| R1253T | Mouse IgG (H&L) | ||||||
| Rabbit | TRITC | 2 mg / US$ 285 | |||||
| R1253TR | Mouse IgG (H&L) | ||||||
| Rabbit | Texas Red | 2 mg / US$ 295 | |||||
| R1253HRP | Mouse IgG (H&L) | ||||||
| Rabbit | HRP | 2 mg / US$ 315 | |||||
| R1253AP | Mouse IgG (H&L) | ||||||
| Rabbit | AP | 1 mg / US$ 325 | |||||
| R1457C2 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy2 | 1 mg / US$ 445 | |||||
| R1457C3 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3 | 1 mg / US$ 445 | |||||
| R1457C35 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy3.5 | 1 mg / US$ 445 | |||||
| R1457C5 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5 | 1 mg / US$ 445 | |||||
| R1457C55 | Mouse IgG (H&L) multi-species ads. | ||||||
| Goat | Cy5.5 | 1 mg / US$ 445 | |||||
| R1405R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Bov, Eq, Hu, Rb, Rt, Sh | ||||||
| Goat | PE | 1 ml / US$ 455 | |||||
| R1403R | Mouse IgG (H&L) F(ab')2 Fragment ads. to Hu serum proteins | ||||||
| Rabbit | PE | 1 ml / US$ 455 | |||||
| R1455R | Mouse IgG (H&L) F(ab')2 Fragment multi-species ads. | ||||||
| Donkey | PE | 1 ml / US$ 455 | |||||
Click here to see all secondary antibodies for 'BM753F Tubulin alpha'.
