BM6052P Cardiotin antibody
see related secondary antibodiessee all 9 Cardiotin products
1 mg / US$ 425
NOVUS BIOLOGICALS
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
PO Box 802 Littleton, CO 80160, USA
E-Mail: novus@novus-biologicals.com
Homepage: http://www.novus-biologicals.com
Quick Overview
Mouse anti Cardiotin SR-4
Product review
Please read our product review about Cardiotin: Antibodies to Cardiotin.
Product Description
Mouse anti Cardiotin SR-4, Presentation: Purified. Product is tested for Frozen sections ( C ), Westernblot / Immunoblot ( WB )
Properties
| Applications | Frozen sections ( C ), Westernblot / Immunoblot ( WB ) |
| Reactivity | Human ( Hu ), Por ( Por ) |
| Presentation | Purified |
| Host | Mouse |
| Isotype | IgM |
| Clone | SR-4 |
| Catalog Number | BM6052P |
| Quantity | 1 mg |
| Price | US$ 425 |
| Delivery | Worldwide |
| Manufacturer | Acris Antibodies GmbH |
| Datasheet | view  BM6052P.pdf |
Datasheet Extract
| Monoclonal Antibody to Cardiotin | |
| Concentration | 1.0 mg/ml |
| Background | Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle α-actinin.
During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes. |
| Immunogen | Derived by fusion of SP2/0-Ag14 mouse myeloma cells with
spleen cells from a BALB/c mouse immunized with cardiotin. |
| Format | State: Liquid purified Ig fraction. BufferSystem: PBS containing 0.09% sodium azide as preservative |
| Applications | Suitable for Immunohistochemistry on frozen sections (1/25-1/50 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent), Immunoblotting (1/25-1/250) and Flow cytometry (1/25-1/50). |
| Specificity | SR-4 recognizes exclusively the 300 kDa cardiotin protein complex by Immunoblotting. Species: Human and swine. |
| Storage | Store the antibody (undiluted) at 2-8°C for one month or (in small aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles. Shelf life: one year from despatch. |
| References | 1. Schaart, G., van der Ven, P. F., and Ramaekers, F. C. (1993).
Characterization of cardiotin, a structural component in the myocard, Eur J Cell Biol 62, 34-48. 2. Schaart, G., Moens, L., Endert, J. M., and Ramaekers, F. C. (1997). Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein, FEBS Lett 403, 168-72. 3. Ausma, J., Wijffels, M., van Eys, G., Koide, M., Ramaekers, F., Allessie, M., and Borgers, M. (1997). Dedifferentiation of atrial cardiomyocytes as a result of chronic atrial fibrillation, Am J Pathol 151, 985-97. 4. Dispersyn, G. D., Geuens, E., Ver Donck, L., Ramaekers, F. C., and Borgers, M. (2001). Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts, Cardiovasc Res 51, 230-40. 5. Ausma, J., Litjens, N., Lenders, M-H., Duimel, H., Mast, F., Wouters, L., Ramaekers, F., Allessie, M., and Borgers, M. (2001). Time course of atrial fibrillation-induced cellular structural remodeling in atria of the goat, J Mol Cell Cardiol 33, 2083-94. 6. Dispersyn, G. D., Mesotten, L., Meuris, B., Maes, A., Mortelmans, L., Flameng, W., Ramaekers, F. C., and Borgers, M. (2002). Dissociation of cardiomyocyte apoptosis and dedifferentiation in infarct border zones, Eur Heart J in press. 7. Ausma, J., van der Velden, H. M., Lenders, M. H., van Ankeren, E. P., Jongsma, H. J., Ramaekers, F. C., Borgers, M., and Allessie, M. A. (2003). Reverse structural and gap-junctional remodeling after prolonged atrial fibrillation in the goat. Circulation |
10 Secondary Antibodies
| Catalog No. | Name / Host | Presentation | Reactivity | ||||
|---|---|---|---|---|---|---|---|
| R1349B | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Biotin | 2 mg / US$ 355 | |||||
| R1349F | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | FITC | 2 mg / US$ 335 | |||||
| R1349T | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | TRITC | 2 mg / US$ 335 | |||||
| R1349TR | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | Texas Red | 2 mg / US$ 355 | |||||
| R1349HRP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | HRP | 2 mg / US$ 365 | |||||
| R1349AP | Mouse IgA + IgG + IgM (H&L) | ||||||
| Goat | AP | 1 mg / US$ 385 | |||||
| SP1018F | Mouse IgM mu chain | ||||||
| Goat | FITC | Ms | 1 mg / US$ 375 | ||||
| SP1018R | Mouse IgM mu chain | ||||||
| Goat | PE | Ms | 0.1 mg / US$ 485 | ||||
| SP1018HRP | Mouse IgM mu chain | ||||||
| Goat | HRP | Ms | 1 mg / US$ 385 | ||||
| SP1018AP | Mouse IgM mu chain | ||||||
| Goat | AP | Ms | 0.8 mg / US$ 425 | ||||
Click here to see all secondary antibodies for 'BM6052P Cardiotin'.
